Abstract
The freeze-etching technique was used to examine the effects of fracturing and etching on the appearance of poly-β-hydroxybutyrate granules from Bacillus cereus. These granules were examined in extracts isolated by hypochlorite or by sonic treatment, and also in fixed and unfixed intact cells; in the latter case they were compared with granules in thin sections of intact cells. After freeze-fracturing, the diameter of the granules in intact cells was between 240 and 720 nm. The granules consisted of a central core, of diameter between 140 and 370 nm, which occupied less than 50% of the volume of the granule and which was either stretched or removed on fracturing; the core was surrounded by an outer coat which may be bounded by a membrane.
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Selected References
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- BOATMAN E. S. OBSERVATIONS ON THE FINE STRUCTURE OF SPHEROPLASTS OF RHODOSPIRILLUM RUBRUM. J Cell Biol. 1964 Feb;20:297–311. doi: 10.1083/jcb.20.2.297. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Dunlop W. F., Robards A. W. Some artifacts of the freeze-etching technique. J Ultrastruct Res. 1972 Aug;40(3):391–400. doi: 10.1016/s0022-5320(72)90109-8. [DOI] [PubMed] [Google Scholar]
- Ellar D. J., Lundgren D. G. Fine structure of sporulation in Bacillus cereus grown in a chemically defined medium. J Bacteriol. 1966 Dec;92(6):1748–1764. doi: 10.1128/jb.92.6.1748-1764.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Ellar D., Lundgren D. G., Okamura K., Marchessault R. H. Morphology of poly-beta-hydroxybutyrate granules. J Mol Biol. 1968 Aug 14;35(3):489–502. doi: 10.1016/s0022-2836(68)80009-9. [DOI] [PubMed] [Google Scholar]
- KELLENBERGER E., RYTER A., SECHAUD J. Electron microscope study of DNA-containing plasms. II. Vegetative and mature phage DNA as compared with normal bacterial nucleoids in different physiological states. J Biophys Biochem Cytol. 1958 Nov 25;4(6):671–678. doi: 10.1083/jcb.4.6.671. [DOI] [PMC free article] [PubMed] [Google Scholar]
- LUNDGREN D. G., ALPER R., SCHNAITMAN C., MARCHESSAULT R. H. CHARACTERIZATION OF POLY-BETA-HYDROXYBUTYRATE EXTRACTED FROM DIFFERENT BACTERIA. J Bacteriol. 1965 Jan;89:245–251. doi: 10.1128/jb.89.1.245-251.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
- LUNDGREN D. G., PFISTER R. M., MERRICK J. M. STRUCTURE OF POLY-BETA-HYDROXYBUTYRIC ACID GRANULES. J Gen Microbiol. 1964 Mar;34:441–446. doi: 10.1099/00221287-34-3-441. [DOI] [PubMed] [Google Scholar]
- Lickfeld K. G., Achterrath M., Hentrich F. [The interpretation of images of cross fractured frozen-etched and shadowed membranes]. J Ultrastruct Res. 1972 Feb;38(3):279–287. doi: 10.1016/s0022-5320(72)90005-6. [DOI] [PubMed] [Google Scholar]
- MERRICK J. M., LUNDGREN D. G., PFISTER R. M. MORPHOLOGICAL CHANGES IN POLY-BETA-HYDROXYBUTYRATE GRANULES ASSOCIATED WITH DECREASED SUSCEPTIBILITY TO ENZYMATIC HYDROLYSIS. J Bacteriol. 1965 Jan;89:234–239. doi: 10.1128/jb.89.1.234-239.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
- PFISTER R. M., LUNDGREN D. G. ELECTRON MICROSCOPY OF POLYRIBOSOMES WITHIN BACILLUS CEREUS. J Bacteriol. 1964 Oct;88:1119–1129. doi: 10.1128/jb.88.4.1119-1129.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
- REYNOLDS E. S. The use of lead citrate at high pH as an electron-opaque stain in electron microscopy. J Cell Biol. 1963 Apr;17:208–212. doi: 10.1083/jcb.17.1.208. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Remsen C. C. The fine structure of frozen-etched Bacillus cereus spores. Arch Mikrobiol. 1966 Sep 8;54(3):266–275. doi: 10.1007/BF00408999. [DOI] [PubMed] [Google Scholar]
- Robards A. W., Parish G. R. Freeze-etching. Lab Pract. 1972 Apr;21(4):254–260. [PubMed] [Google Scholar]
- Sleytr U. Gefrierätzung verschiedener Stämme von Bacillus sphaericus. Arch Mikrobiol. 1970;72(3):238–251. [PubMed] [Google Scholar]
- WILLIAMSON D. H., WILKINSON J. F. The isolation and estimation of the poly-beta-hydroxybutyrate inclusions of Bacillus species. J Gen Microbiol. 1958 Aug;19(1):198–209. doi: 10.1099/00221287-19-1-198. [DOI] [PubMed] [Google Scholar]
- Wang W. S., Lundgren D. G. Poly-beta-hydroxybutyrate in the chemolithotrophic bacterium Ferrobacillus ferrooxidans. J Bacteriol. 1969 Feb;97(2):947–950. doi: 10.1128/jb.97.2.947-950.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
- van Gool A. P., Lambert R., Laudelout H. The fine structure of frozen etched nitrobacter cells. Arch Mikrobiol. 1969;69(4):281–293. doi: 10.1007/BF00408570. [DOI] [PubMed] [Google Scholar]