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. 1973 Nov;116(2):517–526. doi: 10.1128/jb.116.2.517-526.1973

Isolation of a Specialized Lambda Transducing Bacteriophage Carrying the Beta Subunit Gene for Escherichia coli Ribonucleic Acid Polymerase

Joel B Kirschbaum a,1, E Bruce Konrad b
PMCID: PMC285412  PMID: 4583237

Abstract

A heat-inducible lysis-defective λ prophage has been integrated directly into the E. coli chromosome at a site (bfe) very closely linked to the ribonucleic acid polymerase mutation rifd, a dominant rifampin resistance allele. This unusual lysogen has facilitated the isolation of specialized transducing phages conferring rifampin resistance to sensitive cells, and carrying at least the β subunit gene of RNA polymerase in intact form.

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Selected References

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