Abstract
Production of extracellular protease by Bacillus subtilis 168 in a medium containing low concentrations of amino acids is essentially linear, whereas in a medium containing high levels of amino acids the time course of production is biphasic. Cells harvested from the growth medium are capable of secreting enzyme for 30 min in the presence of rifampin, but the appearance of the enzyme is sensitive to chloramphenicol and pactamycin. The protease messenger ribonucleic acid (mRNA), nevertheless, appears to have a short half-life typical of bacterial messengers, and this indicates that these cells contain a relatively large pool of protease-specific mRNA. This pattern of results is identical to that observed previously with B. amyloliquefaciens. Because it has now been found in two distinct organisms, it is concluded that the accumulation of mRNA for extracellular protease, supported by rapid transcription, is a biologically meaningful phenomenon related to extracellular enzyme synthesis rather than aberrant behavior due to a transcriptional control mutation.
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Selected References
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- Both G. W., McInnes J. L., Hanlon J. E., May B. K., Elliott W. H. Evidence for an accumulation of messenger RNA specific for extracellular protease and its relevance to the mechanism of enzyme secretion in bacteria. J Mol Biol. 1972 Jun 20;67(2):199–217. doi: 10.1016/0022-2836(72)90236-7. [DOI] [PubMed] [Google Scholar]
- DONNELLAN J. E., Jr, NAGS E. H., LEVINSON H. S. CHEMICALLY DEFINED, SYNTHETIC MEDIA FOR SPORULATION AND FOR GERMINATION AND GROWTH OF BACILLUS SUBTILIS. J Bacteriol. 1964 Feb;87:332–336. doi: 10.1128/jb.87.2.332-336.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Glenn A. R., Both G. W., McInnes J. L., May B. K., Elliott W. H. Dynamic state of the messenger RNA pool specific for extracellular protease in Bacillus amyloliquefaciens: its relevance to the mechanism of enzyme secretion. J Mol Biol. 1973 Jan 10;73(2):221–230. doi: 10.1016/0022-2836(73)90325-2. [DOI] [PubMed] [Google Scholar]
- Gould A. R., May B. K., Elliott W. H. Accumulation of messenger RNA for extracellular enzymes as a general phenomenon in Bacillus amyloiquefaciens. J Mol Biol. 1973 Jan 10;73(2):213–219. doi: 10.1016/0022-2836(73)90324-0. [DOI] [PubMed] [Google Scholar]
- Millet J. Characterization of proteinases excreted by Bacillus subtilis Marburg strain during sporulation. J Appl Bacteriol. 1970 Mar;33(1):207–219. doi: 10.1111/j.1365-2672.1970.tb05245.x. [DOI] [PubMed] [Google Scholar]
- Welker N. E., Campbell L. L. Unrelatedness of Bacillus amyloliquefaciens and Bacillus subtilis. J Bacteriol. 1967 Oct;94(4):1124–1130. doi: 10.1128/jb.94.4.1124-1130.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]