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. 1973 Nov;116(2):548–561. doi: 10.1128/jb.116.2.548-561.1973

Effect of Isoleucine, Valine, or Leucine Starvation on the Potential for Formation of the Branched-Chain Amino Acid Biosynthetic Enzymes

John J Wasmuth 1, H E Umbarger 1
PMCID: PMC285417  PMID: 4200849

Abstract

The derepression of the isoleucine and valine biosynthetic enzymes in Escherichia coli and Salmonella typhimurium was examined under conditions of restriction of isoleucine, valine, or leucine (the three amino acids needed for multivalent repression of these enzymes). A procedure was used that allowed the measurement of enzyme-forming potential that accumulated during the starvation period, but could not be expressed unless the missing amino acid was supplied. The threonine deaminase (the product of the ilvA gene)-forming potential that accumulated under such conditions was found to be unstable and decayed with a half-life of about 2.5 min (at 37 C). Evidence was obtained that indicates the threonine deaminase-forming potential that accumulates under conditions of isoleucine starvation is in the form of initiated (rifampin-resistant), but uncompleted (actinomycin D-sensitive), messenger ribonucleic acid chains. Furthermore, it appears that a large portion of the threonine deaminase- and dehydrase (the product of the ilvD gene)-forming potential, under such conditions, is in the form of initiated polypeptide chains. Based on these results and results obtained with SuA− strains, a model is presented that explains how the second gene (D) in the ilvADE operon can be partially transcribed and translated under conditions in which there are no completed messenger ribonucleic acids for the gene (A) transcribed before it.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. DAVIS B. D., MINGIOLI E. S. Mutants of Escherichia coli requiring methionine or vitamin B12. J Bacteriol. 1950 Jul;60(1):17–28. doi: 10.1128/jb.60.1.17-28.1950. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. FREUNDLICH M., BURNS R. O., UMBARGER H. E. Control of isoleucine, valine, and leucine biosynthesis. I. Multivalent repression. Proc Natl Acad Sci U S A. 1962 Oct 15;48:1804–1808. doi: 10.1073/pnas.48.10.1804. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Faanes R., Rogers P. Roles of arginine and canavanine in the synthesis and repression of ornithine transcarbamylase by Escherichia coli. J Bacteriol. 1968 Aug;96(2):409–420. doi: 10.1128/jb.96.2.409-420.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. HARTWELL L. H., MAGASANIK B. THE MOLECULAR BASIS OF HISTIDASE INDUCTION IN BACILLUS SUBTILIS. J Mol Biol. 1963 Oct;7:401–420. doi: 10.1016/s0022-2836(63)80033-9. [DOI] [PubMed] [Google Scholar]
  5. Hall B. G., Gallant J. A. On the rate of messenger decay during amino acid starvation. J Mol Biol. 1973 Jan;73(1):121–124. doi: 10.1016/0022-2836(73)90163-0. [DOI] [PubMed] [Google Scholar]
  6. Jacquet M., Kepes A. Initiation, elongation and inactivation of lac messenger RNA in Escherichia coli studied studied by measurement of its beta-galactosidase synthesizing capacity in vivo. J Mol Biol. 1971 Sep 28;60(3):453–472. doi: 10.1016/0022-2836(71)90181-1. [DOI] [PubMed] [Google Scholar]
  7. Kepes A. Transcription and translation in the lactose operon of Escherichia coli studied by in vivo kinetics. Prog Biophys Mol Biol. 1969;19(1):199–236. doi: 10.1016/0079-6107(69)90006-6. [DOI] [PubMed] [Google Scholar]
  8. Klein A., Eisenstadt A., Eisenstadt J., Lengyel P. Inhibition of peptide-chain initiation in Escherichia coli by hydroxylamine and effects on ribonucleic acid synthesis. Biochemistry. 1970 Nov 10;9(23):4542–4549. doi: 10.1021/bi00825a013. [DOI] [PubMed] [Google Scholar]
  9. Kuwano M., Kwan C. N., Apirion D., Schlessinger D. Ribonuclease V of escherichia coli. I. Dependence on ribosomes and translocation. Proc Natl Acad Sci U S A. 1969 Oct;64(2):693–700. doi: 10.1073/pnas.64.2.693. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Kuwano M., Schlessinger D., Morse D. E. Loss of dispensable endonuclease activity in relief of polarity by suA. Nat New Biol. 1971 Jun 16;231(24):214–217. doi: 10.1038/newbio231214a0. [DOI] [PubMed] [Google Scholar]
  11. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  12. Lavallé R., De Hauwer G. Messenger RNA synthesis during amino acid starvation in Escherichia coli. J Mol Biol. 1968 Oct 28;37(2):269–288. doi: 10.1016/0022-2836(68)90267-2. [DOI] [PubMed] [Google Scholar]
  13. Leive L., Kollin V. Controlling EDTA treatment to produce permeable Escherichia coli with normal metabolic processes. Biochem Biophys Res Commun. 1967 Jul 21;28(2):229–236. doi: 10.1016/0006-291x(67)90434-2. [DOI] [PubMed] [Google Scholar]
  14. McLellan W. L., Vogel H. J. Translational repression in the arginine system of Escherichia coli. Proc Natl Acad Sci U S A. 1970 Dec;67(4):1703–1709. doi: 10.1073/pnas.67.4.1703. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Morse D. E., Primakoff P. Relief of polarity in E. coli by "suA". Nature. 1970 Apr 4;226(5240):28–31. doi: 10.1038/226028a0. [DOI] [PubMed] [Google Scholar]
  16. Morse D. E., Yanofsky C. Polarity and the degradation of mRNA. Nature. 1969 Oct 25;224(5217):329–331. doi: 10.1038/224329a0. [DOI] [PubMed] [Google Scholar]
  17. RAMAKRISHNAN T., ADELBERG E. A. REGULATORY MECHANISMS IN THE BIOSYNTHESIS OF ISOLEUCINE AND VALINE. II. IDENTIFICATION OF TWO OPERATOR GENES. J Bacteriol. 1965 Mar;89:654–660. doi: 10.1128/jb.89.3.654-660.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. SOMERVILLE R. L., YANOFSKY C. ON THE TRANSLATION OF THE A GENE REGION OF TRYPTOPHAN MESSENGER RNA. J Mol Biol. 1964 Apr;8:616–619. doi: 10.1016/s0022-2836(64)80019-x. [DOI] [PubMed] [Google Scholar]
  19. Szentirmai A., Umbarger H. E. Isoleucine and valine metabolism of Escherichia coli. XIV. Effect of thiaisoleucine. J Bacteriol. 1968 May;95(5):1666–1671. doi: 10.1128/jb.95.5.1666-1671.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Venetianer P., Berberich M. A., Goldberger R. F. Studies on the size of the messenger-RNA transcribed from the histidine operon during simultaneous and sequential depression. Biochim Biophys Acta. 1968 Aug 23;166(1):124–133. doi: 10.1016/0005-2787(68)90496-6. [DOI] [PubMed] [Google Scholar]

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