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. 1973 Nov;116(2):577–581. doi: 10.1128/jb.116.2.577-581.1973

Genetics and Biochemistry of Pyrimidine Biosynthesis in Bacillus subtilis: Linkage Between Mutations Resulting in a Requirement for Uracil

Raymond J Kelleher Jr a,1, Harry Gooder a
PMCID: PMC285420  PMID: 4200850

Abstract

A number of independently derived uracil-requiring mutant strains of the parent strain 168 were mapped by inter-mutant transformation crosses. Only a few of these mutant strains were found to be transformable. Studies were performed in which these transformable uracil-requiring mutant strains were used as the recipient of deoxyribonucleic acid extracted from phenotypically similar mutant strains. The results yielded data resulting in a fine-structure map in which all mutations were found to be linked in a small region corresponding to that previously published as the uracil region of the Bacillus subtilis genome.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BECKWITH J. R., PARDEE A. B., AUSTRIAN R., JACOB F. Coordination of the synthesis of the enzymes in the pyrimidine pathway of E. coli. J Mol Biol. 1962 Dec;5:618–634. doi: 10.1016/s0022-2836(62)80090-4. [DOI] [PubMed] [Google Scholar]
  2. Bethell M. R., Jones M. E. Molecular size and feedback-regulation characteristics of bacterial asartate transcarbamulases. Arch Biochem Biophys. 1969 Nov;134(2):352–365. doi: 10.1016/0003-9861(69)90294-x. [DOI] [PubMed] [Google Scholar]
  3. Carlton B. C. Fine-structure mapping by transformation in the tryptophan region of Bacillus subtilis. J Bacteriol. 1966 May;91(5):1795–1803. doi: 10.1128/jb.91.5.1795-1803.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Chapman L. F., Nester E. W. Gene-enzyme relationships in histidine biosynthesis in Bacillus subtilis. J Bacteriol. 1969 Mar;97(3):1444–1448. doi: 10.1128/jb.97.3.1444-1448.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Dubnau D., Goldthwaite C., Smith I., Marmur J. Genetic mapping in Bacillus subtilis. J Mol Biol. 1967 Jul 14;27(1):163–185. doi: 10.1016/0022-2836(67)90358-0. [DOI] [PubMed] [Google Scholar]
  6. Hoch J. A., Anagnostopoulos C. Chromosomal location and properties of radiation sensitivity mutations in Bacillus subtilis. J Bacteriol. 1970 Aug;103(2):295–301. doi: 10.1128/jb.103.2.295-301.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Kimhi Y., Magasanik B. Genetic basis of histidine degradation in Bacillus subtilis. J Biol Chem. 1970 Jul 25;245(14):3545–3548. [PubMed] [Google Scholar]
  8. LACKS S., HOTCHKISS R. D. A study of the genetic material determining an enzyme in Pneumococcus. Biochim Biophys Acta. 1960 Apr 22;39:508–518. doi: 10.1016/0006-3002(60)90205-5. [DOI] [PubMed] [Google Scholar]
  9. MASTER R. W. POSSIBLE SYNTHESIS OF POLYRIBONUCLEOTIDES OF KNOWN BASE-TRIPLET SEQUENCES. Nature. 1965 Apr 3;206:93–93. doi: 10.1038/206093b0. [DOI] [PubMed] [Google Scholar]
  10. NESTER E. W., LEDERBERG J. Linkage of genetic units of Bacillus subtilis in DNA transformation. Proc Natl Acad Sci U S A. 1961 Jan 15;47:52–55. doi: 10.1073/pnas.47.1.52. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. NEUMANN J., JONES M. E. END-PRODUCT INHIBITION OF ASPARTATE TRANSCARBAMYLASE IN VARIOUS SPECIES. Arch Biochem Biophys. 1964 Mar;104:438–447. doi: 10.1016/0003-9861(64)90487-4. [DOI] [PubMed] [Google Scholar]
  12. Nasser D., Nester E. W. Aromatic amino acid biosynthesis: gene-enzyme relationships in Bacillus subtilis. J Bacteriol. 1967 Nov;94(5):1706–1714. doi: 10.1128/jb.94.5.1706-1714.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. O'Donovan G. A., Neuhard J. Pyrimidine metabolism in microorganisms. Bacteriol Rev. 1970 Sep;34(3):278–343. doi: 10.1128/br.34.3.278-343.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Rogolsky M. Chromosomal regions which control sporulation in Bacillus subtilis. Can J Microbiol. 1969 Jul;15(7):787–790. doi: 10.1139/m69-137. [DOI] [PubMed] [Google Scholar]
  15. Spizizen J. TRANSFORMATION OF BIOCHEMICALLY DEFICIENT STRAINS OF BACILLUS SUBTILIS BY DEOXYRIBONUCLEATE. Proc Natl Acad Sci U S A. 1958 Oct 15;44(10):1072–1078. doi: 10.1073/pnas.44.10.1072. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. TAYLOR A. L., BECKWITH J. R., PARDEE A. B., AUSTRIAN R., JACOB F. THE CHROMOSOMAL LOCATION OF THE STRUCTURAL GENE FOR OROTIDYLIC ACID PYROPHOSPHORYLASE IN ESCHERICHIA COLI. J Mol Biol. 1964 May;8:771–771. doi: 10.1016/s0022-2836(64)80124-8. [DOI] [PubMed] [Google Scholar]
  17. Taylor A. L., Trotter C. D. Linkage map of Escherichia coli strain K-12. Bacteriol Rev. 1972 Dec;36(4):504–524. doi: 10.1128/br.36.4.504-524.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Whitt D. D., Carlton B. C. Characterization of mutants with single and multiple defects in the tryptophan biosynthetic pathway in Bacillus subtilis. J Bacteriol. 1968 Oct;96(4):1273–1280. doi: 10.1128/jb.96.4.1273-1280.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Wilson G. A., Bott K. F. Nutritional factors influencing the development of competence in the Bacillus subtilis transformation system. J Bacteriol. 1968 Apr;95(4):1439–1449. doi: 10.1128/jb.95.4.1439-1449.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Young F. E., Smith C., Reilly B. E. Chromosomal location of genes regulating resistance to bacteriophage in Bacillus subtilis. J Bacteriol. 1969 Jun;98(3):1087–1097. doi: 10.1128/jb.98.3.1087-1097.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]

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