Abstract
Sixty-two spontaneous mutations have been characterized which reduce the level of expression of catabolite-sensitive operons. These mutations appear to affect either the crp (catabolite gene activator protein) or cya (adenyl cyclase) loci. No new loci have been discovered. Deletions of the cya gene do not remove an essential function. φ80 transducing phage for the cya gene have been used to do recombination and complementation studies on cya mutants.
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Selected References
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- Beckwith J. R., Signer E. R., Epstein W. Transposition of the Lac region of E. coli. Cold Spring Harb Symp Quant Biol. 1966;31:393–401. doi: 10.1101/sqb.1966.031.01.051. [DOI] [PubMed] [Google Scholar]
- Beckwith J., Grodzicker T., Arditti R. Evidence for two sites in the lac promoter region. J Mol Biol. 1972 Aug 14;69(1):155–160. doi: 10.1016/0022-2836(72)90031-9. [DOI] [PubMed] [Google Scholar]
- Davies J., Jacob F. Genetic mapping of the regulator and operator genes of the lac operon. J Mol Biol. 1968 Sep 28;36(3):413–417. doi: 10.1016/0022-2836(68)90165-4. [DOI] [PubMed] [Google Scholar]
- De Crombrugghe B., Chen B., Gottesman M., Pastan I., Varmus H. E., Emmer M., Perlman R. L. Regulation of lac mRNA synthesis in a soluble cell-free system. Nat New Biol. 1971 Mar 10;230(10):37–40. doi: 10.1038/newbio230037a0. [DOI] [PubMed] [Google Scholar]
- Emmer M., deCrombrugghe B., Pastan I., Perlman R. Cyclic AMP receptor protein of E. coli: its role in the synthesis of inducible enzymes. Proc Natl Acad Sci U S A. 1970 Jun;66(2):480–487. doi: 10.1073/pnas.66.2.480. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Epstein W., Kim B. S. Potassium transport loci in Escherichia coli K-12. J Bacteriol. 1971 Nov;108(2):639–644. doi: 10.1128/jb.108.2.639-644.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Eron L., Block R. Mechanism of initiation and repression of in vitro transcription of the lac operon of Escherichia coli. Proc Natl Acad Sci U S A. 1971 Aug;68(8):1828–1832. doi: 10.1073/pnas.68.8.1828. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hofnung M., Schwartz M., Hatfield D. Complementation studies in the maltose-A region of the Escherichia coli K12 genetic map. J Mol Biol. 1971 Nov 14;61(3):681–694. doi: 10.1016/0022-2836(71)90072-6. [DOI] [PubMed] [Google Scholar]
- Miller J. H., Ippen K., Scaife J. G., Beckwith J. R. The promoter-operator region of the lac operon of Escherichia coli. J Mol Biol. 1968 Dec;38(3):413–420. doi: 10.1016/0022-2836(68)90395-1. [DOI] [PubMed] [Google Scholar]
- Ohlsson B. M., Strigini P. F., Beckwith J. R. Allelic amber and ochre suppressors. J Mol Biol. 1968 Sep 14;36(2):209–218. doi: 10.1016/0022-2836(68)90376-8. [DOI] [PubMed] [Google Scholar]
- Perlman R. L., Pastan I. Pleiotropic deficiency of carbohydrate utilization in an adenyl cyclase deficient mutant of Escherichia coli. Biochem Biophys Res Commun. 1969 Sep 24;37(1):151–157. doi: 10.1016/0006-291x(69)90893-6. [DOI] [PubMed] [Google Scholar]
- Schwartz D. O., Beckwith J. R. Mutagens which cause deletions in Escherichia coli. Genetics. 1969 Feb;61(2):371–376. doi: 10.1093/genetics/61.2.371. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Schwartz M. Location of the maltose A and B loci on the genetic map of Escherichia coli. J Bacteriol. 1966 Oct;92(4):1083–1089. doi: 10.1128/jb.92.4.1083-1089.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Schwartz M. Sur l'existence chez Escherichia coli K 12 d'une régulation commune à la biosynthèse des récepteurs du bactériophage et au métabolisme du maltose. Ann Inst Pasteur (Paris) 1967 Nov;113(5):685–704. [PubMed] [Google Scholar]
- Yokota T., Gots J. S. Requirement of adenosine 3', 5'-cyclic phosphate for flagella formation in Escherichia coli and Salmonella typhimurium. J Bacteriol. 1970 Aug;103(2):513–516. doi: 10.1128/jb.103.2.513-516.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Zubay G., Gielow L., Englesberg E. Cell-free studies on the regulation of the arabinose operon. Nat New Biol. 1971 Oct 6;233(40):164–165. doi: 10.1038/newbio233164a0. [DOI] [PubMed] [Google Scholar]
- Zubay G., Schwartz D., Beckwith J. Mechanism of activation of catabolite-sensitive genes: a positive control system. Proc Natl Acad Sci U S A. 1970 May;66(1):104–110. doi: 10.1073/pnas.66.1.104. [DOI] [PMC free article] [PubMed] [Google Scholar]
