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. Author manuscript; available in PMC: 2011 May 1.
Published in final edited form as: Bone. 2010 Feb 1;46(5):1306–1315. doi: 10.1016/j.bone.2010.01.380

Fig. 3.

Fig. 3

In vivo monitoring of cherubism Sh3bp2KI/KI osteoblast cultures using Col1a1 promoter-driven GFP. Sh3bp2+/+ and Sh3bp2KI/KI mice were crossed with transgenic mice overexpressing GFP driven by either 3.6-kb or 2.3-kb Col1a1 promoters. Frozen sections (5 μm) of femurs from 10-week-old mice showed increased numbers of 3.6-kb Col1a1 GFP positive cells (3.6GFP) in Sh3bp2KI/KI mice (A), but decreased numbers of 2.3-kb Col1a1 GFP positive cells (2.3GFP) (B). (Student's t-test; n=6 for pOBCol3.6GFPtpz; n=4 for pOBCol2.3GFPemd, *p<0.05). 3.6-kb Col1a1 promoter in osteocytes from femoral cortex of Sh3bp2KI/KI but not of Sh3bp2+/+ mice was active (C). Trab: trabecular bone. Cort: Cortical bone.