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. 1973 Nov;116(2):710–718. doi: 10.1128/jb.116.2.710-718.1973

Messenger Ribonucleic Acid Synthesis and Degradation in Escherichia coli During Inhibition of Translation

Martin L Pato 1,2, Peter M Bennett 1,2,1, Kaspar Von Meyenburg 1,2
PMCID: PMC285436  PMID: 4583248

Abstract

Various aspects of the coupling between the movement of ribosomes along messenger ribonucleic acids (mRNA) and the synthesis and degradation of mRNA have been investigated. Decreasing the rate of movement of ribosomes along an mRNA does not affect the rate of movement of some, and possibly most, of the RNA polymerases transcribing the gene coding for that mRNA. Inhibiting translation with antibiotics such as chloramphenicol, tetracycline, or fusidic acid protects extant mRNA from degradation, presumably by immobilizing ribosomes, whereas puromycin exposes mRNA to more rapid degradation than normal. The promoter distal (3′) portion of mRNA, synthesized after ribosomes have been immobilized by chloramphenicol on the promoter proximal (5′) portion of the mRNA, is subsequently degraded.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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