Abstract
A number of mutations affecting the transport of hexose phosphates in Escherichia coli were ordered within the uhp locus. Three-point crosses by transduction or conjugation allowed the ordering of the alleles relative to the adjacent pyrE marker. The same linear map was obtained by both methods. This, combined with the regulatory properties of revertants of these mutants, allowed a tentative identification of two genes, one presumably coding for the transport system (uhpT) and the other(s) specifying a regulatory element (uhpR). The order of these is pyrE-uhpT-uhpR. Mutants exhibiting constitutive expression of the transport system were isolated. This behavior is genetically linked to the uhp locus, but more precise localization was not possible.
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Selected References
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- Dietz G. W., Heppel L. A. Studies on the uptake of hexose phosphates. II. The induction of the glucose 6-phosphate transport system by exogenous but not by endogenously formed glucose 6-phosphate. J Biol Chem. 1971 May 10;246(9):2885–2890. [PubMed] [Google Scholar]
- FRAENKEL D. G., FALCOZ-KELLY F., HORECKER B. L. THE UTILIZATION OF GLUCOSE 6-PHOSPHATE BY GLUCOKINASELESS AND WILD-TYPE STRAINS OF ESCHERICHIA COLI. Proc Natl Acad Sci U S A. 1964 Nov;52:1207–1213. doi: 10.1073/pnas.52.5.1207. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Ferenci T., Kornberg H. L., Smith Janet. Isolation and properties of a regulatory mutant in the hexose phosphate transport system of Escherichia coli. FEBS Lett. 1971 Mar 5;13(3):133–136. doi: 10.1016/0014-5793(71)80218-1. [DOI] [PubMed] [Google Scholar]
- Fraenkel D. G. The accumulation of glucose 6-phosphate from glucose and its effect in an Escherichia coli mutant lacking phosphoglucose isomerase and glucose 6-phosphate dehydrogenase. J Biol Chem. 1968 Dec 25;243(24):6451–6457. [PubMed] [Google Scholar]
- Friedberg I. Localization of phosphoglucose isomerase in Escherichia coli and its relation to the induction of the hexose phosphate transport system. J Bacteriol. 1972 Dec;112(3):1201–1205. doi: 10.1128/jb.112.3.1201-1205.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kadner R. J., Winkler H. H. Isolation and characterization of mutations affecting the transport of hexose phosphates in Escherichia coli. J Bacteriol. 1973 Feb;113(2):895–900. doi: 10.1128/jb.113.2.895-900.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kornberg H. L., Smith J. Genetic control of hexose phosphate uptake by Escherichia coli. Nature. 1969 Dec 27;224(5226):1261–1262. doi: 10.1038/2241261a0. [DOI] [PubMed] [Google Scholar]
- Pogell B. M., Maity B. R., Frumkin S., Shapiro S. Induction of an active transport system for glucose 6-phosphate in Escherichia coli. Arch Biochem Biophys. 1966 Sep 26;116(1):406–415. doi: 10.1016/0003-9861(66)90047-6. [DOI] [PubMed] [Google Scholar]
- Winkler H. H. A hexose-phosphate transport system in Escherichia coli. Biochim Biophys Acta. 1966 Mar 28;117(1):231–240. doi: 10.1016/0304-4165(66)90170-x. [DOI] [PubMed] [Google Scholar]
- Winkler H. H. Compartmentation in the induction of the hexose-6-phosphate transport system of Escherichia coli. J Bacteriol. 1970 Feb;101(2):470–475. doi: 10.1128/jb.101.2.470-475.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Winkler H. H. Kinetics of exogenous induction of the hexose-6-phosphate transport system of Escherichia coli. J Bacteriol. 1971 Jul;107(1):74–78. doi: 10.1128/jb.107.1.74-78.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]