Skip to main content
NCBI home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1973 Nov;116(2):847–856. doi: 10.1128/jb.116.2.847-856.1973

Glucosylation of Lipopolysaccharide in Salmonella: Mutants Negative for O Antigen Factor 1221

P Helena Mäkelä a
PMCID: PMC285455  PMID: 4583253

Abstract

In salmonella O group B, the O antigen factor 122 is created by glucosylation at C4 of the galactose units of the O side chains; phage-determined glucosylation at C6 of these galactose units yields factor 1. 122-negative mutants were isolated from 122+ (stable) parents (genetically oafR+st). All 20 mutants studied were stable in respect of their 122 character. In eight of them, lysogenization by phage P22 resulted in the appearance of the 122 factor—these were interpreted to be defective in the enzyme(s) needed to synthesize the glucose-lipid intermediate that participates in both 122- and 1-specific glucosylation; the corresponding cistron was termed oafE. This “complementation” assay also demonstrated that the P22 genome can determine the synthesis of this glucose-lipid intermediate. The 122 character in the oafE− mutants became variable after P22 lysogenization, corresponding to factor 1 variation normally determined by this phage. In the remaining 12 mutants, P22 caused the appearance of a variable factor 1, but not of 122. The lesion in all the 122 mutants was mapped close to the gene purE at min 19 of the Salmonella map. This is the same area where the locus oafR that controls the variation (stable or variable, + or −) of 122 had been previously mapped. The results suggest the presence of a 122 glucosylation operon in this region.

Full text

PDF
847

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bagdian G., Mäkelä P. H. Antigenic conversion by phage P27. I. Mapping of the prophage attachment site on the Salmonella chromosome. Virology. 1971 Feb;43(2):403–411. doi: 10.1016/0042-6822(71)90312-6. [DOI] [PubMed] [Google Scholar]
  2. Cerdá-Olmedo E., Hanawalt P. C., Guerola N. Mutagenesis of the replication point by nitrosoguanidine: map and pattern of replication of the Escherichia coli chromosome. J Mol Biol. 1968 May 14;33(3):705–719. doi: 10.1016/0022-2836(68)90315-x. [DOI] [PubMed] [Google Scholar]
  3. FUKAZAWA Y., HARTMAN P. E. A P22 BACTERIOPHAGE MUTANT DEFECTIVE IN ANTIGEN CONVERSION. Virology. 1964 Jun;23:279–283. doi: 10.1016/0042-6822(64)90296-x. [DOI] [PubMed] [Google Scholar]
  4. HARTMAN P. E., LOPER J. C., SERMAN D. Fine structure mapping by complete transduction between histidine-requiring Salmonella mutants. J Gen Microbiol. 1960 Apr;22:323–353. doi: 10.1099/00221287-22-2-323. [DOI] [PubMed] [Google Scholar]
  5. Hellerqvist C. G., Larm O., Lindberg B., Holme T., Lindberg A. A. Structural studies on the O-specific side chains of the cell wall lipopolysaccharide from Salmonella bredeney. Acta Chem Scand. 1969;23(7):2217–2222. doi: 10.3891/acta.chem.scand.23-2217. [DOI] [PubMed] [Google Scholar]
  6. Kuo T. T., Stocker B. A. ES18, a general transducing phage for smooth and nonsmooth Salmonella typhimurium. Virology. 1970 Nov;42(3):621–632. doi: 10.1016/0042-6822(70)90308-9. [DOI] [PubMed] [Google Scholar]
  7. Kuo T. T., Stocker B. A. Suppression of proline requirement of proA and proAB deletion mutants in Salmonella typhimurium by mutation to arginine requirement. J Bacteriol. 1969 May;98(2):593–598. doi: 10.1128/jb.98.2.593-598.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. MAKELA P. H. HFR males in Salmonella abony. Genetics. 1963 Mar;48:423–429. doi: 10.1093/genetics/48.3.423. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. MEYNELL G. G. Salmonella enteriditis as a genetic donor in intraspecific and interspecific crosses initiated by colicine factors. J Gen Microbiol. 1962 Apr;28:169–176. doi: 10.1099/00221287-28-1-169. [DOI] [PubMed] [Google Scholar]
  10. Mäkelä P. H., Jahkola M., Lüderitz O. A new gene cluster rfe concerned with the biosynthesis of Salmonella lipopolysaccharide. J Gen Microbiol. 1970 Jan;60(1):91–106. doi: 10.1099/00221287-60-1-91. [DOI] [PubMed] [Google Scholar]
  11. Mäkelä P. H., Mäkelä O. Salmonella antigen 12-2: genetics of form variation. Ann Med Exp Biol Fenn. 1966;44(2):310–317. [PubMed] [Google Scholar]
  12. Mäkelä P. H., Ziegler L. An F' episome of Salmonella abony. Acta Pathol Microbiol Scand. 1966;67(4):547–554. doi: 10.1111/apm.1966.67.4.547. [DOI] [PubMed] [Google Scholar]
  13. Nikaido H., Nikaido K., Nakae T., Mäkelä P. H. Glucosylation of lipopolysaccharide in Salmonella: biosynthesis of O antigen factor 12 2 . I. Over-all reaction. J Biol Chem. 1971 Jun 25;246(12):3902–3911. [PubMed] [Google Scholar]
  14. SMITH S. M., STOCKER B. A. Colicinogeny and recombination. Br Med Bull. 1962 Jan;18:46–51. doi: 10.1093/oxfordjournals.bmb.a069934. [DOI] [PubMed] [Google Scholar]
  15. STOCKER B. A., STAUB A. M., TENELLI R., KOPACKA B. [Immunochemical study on Salmonella. VI. Study of antigen 1 present on 2 Salmonella of the B and E4 groups]. Ann Inst Pasteur (Paris) 1960 Apr;98:505–523. [PubMed] [Google Scholar]
  16. Sanderson K. E. Linkage map of Salmonella typhimurium, edition IV. Bacteriol Rev. 1972 Dec;36(4):558–586. doi: 10.1128/br.36.4.558-586.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Sanderson K. E., Ross H., Ziegler L., Mäkelä P. H. F + , Hfr, and F' strains of Salmonella typhimurium and Salmonella abony. Bacteriol Rev. 1972 Dec;36(4):608–637. doi: 10.1128/br.36.4.608-637.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. TINELLI R., STAUB A. M. [Immunochemical study on Salmonella. 7. Study of the products formed by the controlled acid hydrolysis of the polysaccharide extracted from S. typhi. Part 1. Analysis of antigen O: 12 in the Kauffmann-White scheme]. Bull Soc Chim Biol (Paris) 1960;42:583–599. [PubMed] [Google Scholar]
  19. Takeshita M., Mäkelä P. H. Glucosylation of lipopolysaccharide in Salmonella: biosynthesis of O antigen factor 12 2 . 3. The presence of 12 2 determinants in haptenic polysaccharides. J Biol Chem. 1971 Jun 25;246(12):3920–3927. [PubMed] [Google Scholar]
  20. Wilkinson R. G., Gemski P., Jr, Stocker B. A. Non-smooth mutants of Salmonella typhimurium: differentiation by phage sensitivity and genetic mapping. J Gen Microbiol. 1972 May;70(3):527–554. doi: 10.1099/00221287-70-3-527. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES