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. 1973 Nov;116(2):944–949. doi: 10.1128/jb.116.2.944-949.1973

Camphor Plasmid-Mediated Chromosomal Transfer in Pseudomonas putida

M Shaham a, A M Chakrabarty a,1, I C Gunsalus a
PMCID: PMC285467  PMID: 4745436

Abstract

Camphor-utilizing strains of Pseudomonas putida have been shown to carry the genetic information required for camphor degradation on a plasmid. The plasmid-carrying strains can serve as donors of both plasmid-borne and chromosomal genes. As recipients, plasmid-deleted strains are much superior to those carrying the camphor pathway genes. The transfer frequency of chromosomal, but not plasmid-borne, genes is markedly enhanced if the donor cells are irradiated with ultraviolet light followed by 3-h of growth on a rich medium in the dark. Recombinants selected for prototrophy are stable and most acquire the camphor (CAM) plasmid concomitantly; only a few of the Cam+ recombinants inherit the donor's ability to transfer chromosomal genes at a high frequency. Transfer-defective mutations occur on the CAM plasmid, affecting both CAM and chromosomal gene transfer.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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