Abstract
Lysozyme, used to prepare osmotically fragile cells, combines with ribosomal subunits unless salt is present in excess of 50 mM. The binding of lysozyme to precursor ribonucleoprotein particles can result in their conversion to particles which sediment as mature ribosomes.
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Selected References
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- Godson G. N., Sinsheimer R. L. Use of Brij lysis as a general method to prepare polyribosomes from Escherichia coli. Biochim Biophys Acta. 1967 Dec 19;149(2):489–495. doi: 10.1016/0005-2787(67)90176-1. [DOI] [PubMed] [Google Scholar]
- McFARLANE A. S. Efficient trace-labelling of proteins with iodine. Nature. 1958 Jul 5;182(4627):53–53. doi: 10.1038/182053a0. [DOI] [PubMed] [Google Scholar]
- Patterson D., Weinstein M., Nixon R., Gillespie D. Interaction of ribosomes and the cell envelope of Escherichia coli mediated by lysozyme. J Bacteriol. 1970 Feb;101(2):584–591. doi: 10.1128/jb.101.2.584-591.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Ron E. Z., Kohler R. E., Davis B. D. Polysomes extracted from Escherichia coli by freeze-thaw-lysozyme lysis. Science. 1966 Sep 2;153(3740):1119–1120. doi: 10.1126/science.153.3740.1119. [DOI] [PubMed] [Google Scholar]
- Tremblay G. Y., Daniels M. J., Schaechter M. Isolation of a cell membrane-DNA-nascent RNA complex from bacteria. J Mol Biol. 1969 Feb 28;40(1):65–76. doi: 10.1016/0022-2836(69)90296-4. [DOI] [PubMed] [Google Scholar]