Abstract
A Saccharomyces cerevisiae conditional mutant, LK 181, is described which grows at 37 C only when supplemented with a saturated fatty acid of 12 to 14 carbon atoms chain length. At 22 C, however, no fatty acid supplementation is required for growth. The fatty acid concentration required for optimal growth at 37 C is about four times lower for LK 181 than for fatty acid synthetase-deficient mutants. In contrast to all fatty acid synthetase mutants so far examined, mutant LK 181 cannot grow with palmitic acid. The addition of palmitic, palmitoleic, or oleic acid to the culture medium prevents LK 181 growth at temperatures between 22 and 37 C. In vivo as well as in vitro, cellular de novo fatty acid biosynthesis from acetate is unimpaired in this mutant. It is suggested that endogenously synthesized fatty acids, due to their chain lengths of 16 and more carbon atoms, cannot supplement the mutant LK 181. It is concluded that the exogeneously supplied fatty acids act as allosteric effectors for a mutationally altered cellular protein to restore its biological function at elevated temperatures, rather than as a substitute for endogenously synthesized long-chain fatty acids.
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- Burkl G., Castorph H., Schweizer E. Mapping of a complex gene locus coding for part of the Saccharomyces cerevisiae fatty acid synthetase multienzyme complex. Mol Gen Genet. 1972;119(4):315–322. doi: 10.1007/BF00272089. [DOI] [PubMed] [Google Scholar]
- Henry S. A., Fogel S. Saturated fatty acid mutants in yeast. Mol Gen Genet. 1971;113(1):1–19. doi: 10.1007/BF00335003. [DOI] [PubMed] [Google Scholar]
- Klein K., Steinberg R., Fiethen B., Overath P. Fatty acid degradation in Escherichia coli. An inducible system for the uptake of fatty acids and further characterization of old mutants. Eur J Biochem. 1971 Apr;19(3):442–450. doi: 10.1111/j.1432-1033.1971.tb01334.x. [DOI] [PubMed] [Google Scholar]
- Kühn L., Castorph H., Schweizer E. Gene linkage and gene-enzyme relations in the fatty-acid-synthetase system of Saccharomyces cerevisiae. Eur J Biochem. 1972 Jan 21;24(3):492–497. doi: 10.1111/j.1432-1033.1972.tb19711.x. [DOI] [PubMed] [Google Scholar]
- Munkres K. D., Woodward D. O. On the genetics of enzyme locational specificity. Proc Natl Acad Sci U S A. 1966 May;55(5):1217–1224. doi: 10.1073/pnas.55.5.1217. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Orme T. W., McIntyre J., Lynen F., Kühn L., Schweizer E. Fatty-acid elongation in a mutant of Saccharomyces cerevisiae deficient in fatty-acid synthetase. Eur J Biochem. 1972 Jan 21;24(3):407–415. doi: 10.1111/j.1432-1033.1972.tb19700.x. [DOI] [PubMed] [Google Scholar]
- Pugh E. L., Sauer F., Waite M., Toomey R. E., Wakil S. J. Studies on the mechanism of fatty acid synthesis. 13. The role of beta-hydroxy acids in the synthesis of palmitate and cis vaccenate by the Escherichia coli enzyme system. J Biol Chem. 1966 Jun 10;241(11):2635–2643. [PubMed] [Google Scholar]
- Schweizer E., Bolling H. A Saccharomyces cerevisiae mutant defective in saturated fatty acid biosynthesis. Proc Natl Acad Sci U S A. 1970 Oct;67(2):660–666. doi: 10.1073/pnas.67.2.660. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Schweizer E., Duba C., Chan W., Lynen F. Meccanismo di biosintesi degli acidi grassi. Minerva Pediatr. 1966 Apr 14;18(13):642–646. [PubMed] [Google Scholar]
- Schweizer E., Kühn L., Castorph H. A new gene cluster in yeast: the fatty acid synthetase system. Hoppe Seylers Z Physiol Chem. 1971 Mar;352(3):377–384. doi: 10.1515/bchm2.1971.352.1.377. [DOI] [PubMed] [Google Scholar]
- Schweizer E., Lerch I., Kroeplin-Rueff L., Lynen F. Fatty acyl transferase. Characterization of the enzyme as part of the yeast fatty acid synthetase complex by the use of radioactively labeled coenzyme A. Eur J Biochem. 1970 Sep;15(3):472–482. doi: 10.1111/j.1432-1033.1970.tb01030.x. [DOI] [PubMed] [Google Scholar]
- Sumper M., Oesterhelt D., Riepertinger C., Lynen F. Die Synthese verschiedener Carbonsäuren durch den Multienzymkomplex der Fettsäuresynthese aus Hefe und die Erklärung ihrer Bildung. Eur J Biochem. 1969 Sep;10(2):377–387. doi: 10.1111/j.1432-1033.1969.tb00701.x. [DOI] [PubMed] [Google Scholar]
- WAGNER R. P., BERGQUIST A. Synthesis of valine and isoleucine in the presence of a particulate cell fraction of Neurospora. Proc Natl Acad Sci U S A. 1963 Jun;49:892–897. doi: 10.1073/pnas.49.6.892. [DOI] [PMC free article] [PubMed] [Google Scholar]