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. 1974 Feb;117(2):373–378. doi: 10.1128/jb.117.2.373-378.1974

C4-Dicarboxylate Transport in Bacillus subtilis Studied with 3-Fluoro-l-Erythro-Malate as a Substrate

Klaus Willecke 1, Rita Lange 1
PMCID: PMC285523  PMID: 4204434

Abstract

Bacillus subtilis cells grown in yeast extract medium accumulated 3-fluoro-l-erythro-[1,2-14C2]malate more than 30-fold from the surrounding medium. No metabolic products derived from 3-fluoro-l-erythro-malate could be detected in these cells. l-Malate competitively inhibited transport of 3-fluoro-l-erythro-malate. This malate analogue was itself a competitive inhibitor of l-malate uptake. Cells that had been grown in yeast extract supplemented with 5 mM l-malate showed a 10-fold increased affinity towards 3-fluoro-l-erythro-malate relative to cells grown in yeast extract medium with no added malate. Our results suggest that two transport systems for l-malate can be induced in B. subtilis. The first of these systems seems to effect uptake of C4-dicarboxylates (l-malate, succinate, and fumarate) in yeast extract medium. The second transport system (or possibly a modification of the first transport system) seems to be induced by addition of l-malate to this medium and is also functioning in malate minimal medium.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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