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. 1974 Feb;117(2):468–476. doi: 10.1128/jb.117.2.468-476.1974

Isolation of Specialized Transducing Bacteriophages for Gluconate 6-Phosphate Dehydrogenase (gnd) of Escherichia coli

Richard E Wolf Jr 1, D G Fraenkel 1
PMCID: PMC285536  PMID: 4590471

Abstract

Specialized transducing phages for gluconate 6-phosphate dehydrogenase (gnd), a constitutive enzyme in Escherichia coli, have been isolated using a method previously described for other genes. The gnd-his region, carried on an F′ episome, was first transposed to tonB. Rare phages carrying gnd were selected, by transduction, from φ80 lysogens of these strains; one phage also carried his (φ80gndhis). From the transductants, high-frequency transducing lysates were obtained; low multiplicity of infection then yielded defective lysogens. tonB deletion analysis of the φ80dgndhis lysogen shows the order of genes in the prophage to be imm80...hisOGD...gnd; according to a marker rescue experiment most phage late genes have been replaced by bacterial deoxyribonucleic acid. A heat-inducible, lysis-defective λ-φ80 hybrid derivative of φ80dgndhis has been prepared.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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