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. 1974 Feb;117(2):560–567. doi: 10.1128/jb.117.2.560-567.1974

Ethylene Glycol-Induced Alteration of Conidial Germination in Neurospora crassa

W K Bates 1, J F Wilson 1
PMCID: PMC285546  PMID: 4359649

Abstract

In nutrient medium containing 3.22 M ethylene glycol or glycerol, conidia of Neurospora crassa grow as single cells, without forming the germ tubes characteristic of normal morphological germination. Ethylene glycol is more effective than glycerol in producing this response. After growth in ethylene glycol medium for a suitable time, the cells are easily disrupted by an abrupt decrease in osmotic pressure. Osmotic disruption yields intact nuclei and mitochondria, although mitochondrial fractions obtained in this way show significantly reduced concentrations of cytochromes c + c1, as compared to those observed for comparable fractions obtained from vegetative hyphae. Cell cultures gradually adapted to lower concentrations of the glycol show a much higher degree of synchrony in the formation of germ tubes than do untreated conidia.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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