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. 1974 Feb;117(2):675–680. doi: 10.1128/jb.117.2.675-680.1974

Divergent Orientation of Transcription from the Arginine Gene ECBH Cluster of Escherichia coli

Chandrakant J Panchal 1, Sourindra N Bagchee 1, Arabinda Guha 1
PMCID: PMC285559  PMID: 4590481

Abstract

Ribonucleic acid (RNA) isolated from Escherichia coli W3350 (F, argE+C+B+H+), in the absence of l-arginine, hybridizes with the separated leftward (l) and rightward (r) transcribing strands of the arginine transducing phage hφ80dargE+C+B+H+ppc+immλcI857 deoxyribonucleic acid (DNA) with a ratio of 30:70, respectively. In the presence of l-arginine and its intermediates, l-ornithine and l-citrulline, RNA transcriptions from both the strands of the argECBH cluster were repressed. The derepressed RNA, when hybridized with the separated strands of hφ80dargEC-I immλ phage DNA (the arginine genes are inversely inserted in this phage), which has a deletion in gene E and extends to gene C of the argECBH cluster, showed no leftward transcription, whereas the rightward transcription was reduced to about 40% of that when the DNA carrying the entire ECBH cluster was used for hybridization. The hybridization results thus demonstrate that (i) the regulation of the argECBH gene cluster in E. coli is under transcriptional control, (ii) the orientation of transcription is divergent, (iii) E gene transcribes anticlockwise, whereas the rest of the genes, C, B, and H, transcribe clockwise, and (iv) the position of the promoter(s) and operator(s) is located between the E and C genes of the argECBH cluster.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bollon A. P., Vogel H. J. Regulation of argE-argH expression with arginine derivatives in Escherichia coli: extreme non-uniformity of repression and conditional repressive action. J Bacteriol. 1973 May;114(2):632–640. doi: 10.1128/jb.114.2.632-640.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Cunin Raymond, Glansdorff Nicolas. Messenger RNA from arginine and phosphoenolpyruvate carboxylase genes in arg R+ and arg R(-) strains of E. coli K-12. FEBS Lett. 1971 Oct 15;18(1):135–137. doi: 10.1016/0014-5793(71)80428-3. [DOI] [PubMed] [Google Scholar]
  3. Elseviers D., Cunin R., Glansdorff N. Control regions within the argECBH gene cluster of Escherichia coli K12. Mol Gen Genet. 1972;117(4):349–366. doi: 10.1007/BF00333028. [DOI] [PubMed] [Google Scholar]
  4. GORINI L., GUNDERSEN W., BURGER M. Genetics of regulation of enzyme synthesis in the arginine biosynthetic pathway of Escherichia coli. Cold Spring Harb Symp Quant Biol. 1961;26:173–182. doi: 10.1101/sqb.1961.026.01.022. [DOI] [PubMed] [Google Scholar]
  5. Glansdorf N., Sand G. Coordination of enzyme synthesis in the arginine pathway of Escherichia coli K-12. Biochim Biophys Acta. 1965 Oct 11;108(2):308–311. doi: 10.1016/0005-2787(65)90016-x. [DOI] [PubMed] [Google Scholar]
  6. Guha A. Divergent orientation of transcription from the biotin locus of Escherichia coli. J Mol Biol. 1971 Feb 28;56(1):53–62. doi: 10.1016/0022-2836(71)90083-0. [DOI] [PubMed] [Google Scholar]
  7. Guha A., Szybalski W., Salser W., Geiduschek E. P., Pulitzer J. F., Bolle A. Controls and polarity of transcription during bacteriophage T4 development. J Mol Biol. 1971 Jul 28;59(2):329–349. doi: 10.1016/0022-2836(71)90054-4. [DOI] [PubMed] [Google Scholar]
  8. Hradecna Z., Szybalski W. Electron micrographic maps of deletions and substitutions in the genomes of transducing coliphages lambda dg and lambda bio. Virology. 1969 Jul;38(3):473–477. doi: 10.1016/0042-6822(69)90160-3. [DOI] [PubMed] [Google Scholar]
  9. Jacoby G. A. Control of the argECBH cluster in Escherichia coli. Mol Gen Genet. 1972;117(4):337–348. doi: 10.1007/BF00333027. [DOI] [PubMed] [Google Scholar]
  10. Jacoby G. A., Gorini L. A unitary account of the repression mechanism of arginine biosynthesis in Escherichia coli. I. The genetic evidence. J Mol Biol. 1969 Jan 14;39(1):73–87. doi: 10.1016/0022-2836(69)90334-9. [DOI] [PubMed] [Google Scholar]
  11. MAAS W. K. Studies on repression of arginine biosynthesis in Escherichia coli. Cold Spring Harb Symp Quant Biol. 1961;26:183–191. doi: 10.1101/sqb.1961.026.01.023. [DOI] [PubMed] [Google Scholar]
  12. NYGAARD A. P., HALL B. D. FORMATION AND PROPERTIES OF RNA-DNA COMPLEXES. J Mol Biol. 1964 Jul;9:125–142. doi: 10.1016/s0022-2836(64)80095-4. [DOI] [PubMed] [Google Scholar]
  13. Taylor A. L., Trotter C. D. Linkage map of Escherichia coli strain K-12. Bacteriol Rev. 1972 Dec;36(4):504–524. doi: 10.1128/br.36.4.504-524.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]

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