Abstract
A technique was developed for measuring 14CO2 uptake by chemolithotrophic bacteria directly in pyritic materials associated with coal and coal refuse. There was good correlation between 14CO2 uptake, as determined by this technique, and the most probable number of iron-oxidizing bacteria. Maximal 14CO2 uptake occurred in coal refuse material 2 to 3 years old, and only slight incorporation was demonstrated in fresh material or material 40 years old. Samples taken from the surface of the coal refuse pile always demonstrated maximal 14CO2 uptake, and in most samples, only slight activity was demonstrated at depths below 8 to 10 cm. Optimal uptake of 14CO2 by natural samples occurred at 20 to 30 C and at a moisture content of between 23 and 35%. In addition to chemolithotrophic bacteria, heterotrophic fungi and yeasts were also routinely isolated in high numbers from acidic coal refuse. In contrast, acidophilic, heterotrophic bacteria were either absent or present in low numbers in such acidic samples.
Full text
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Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- ALLEN M. B. Studies with Cyanidium caldarium, an anomalously pigmented chlorophyte. Arch Mikrobiol. 1959;32(3):270–277. doi: 10.1007/BF00409348. [DOI] [PubMed] [Google Scholar]
- COLMER A. R., TEMPLE K. L., HINKLE M. E. An iron-oxidizing bacterium from the acid drainage of some bituminous coal mines. J Bacteriol. 1950 Mar;59(3):317–328. doi: 10.1128/jb.59.3.317-328.1950. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Colmer A. R., Hinkle M. E. The Role of Microorganisms in Acid Mine Drainage: A Preliminary Report. Science. 1947 Sep 19;106(2751):253–256. doi: 10.1126/science.106.2751.253. [DOI] [PubMed] [Google Scholar]
- Fliermans C. B., Brock T. D. Ecology of sulfur-oxidizing bacteria in hot acid soils. J Bacteriol. 1972 Aug;111(2):343–350. doi: 10.1128/jb.111.2.343-350.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kelly D. P. Autotrophy: concepts of lithotrophic bacteria and their organic metabolism. Annu Rev Microbiol. 1971;25:177–210. doi: 10.1146/annurev.mi.25.100171.001141. [DOI] [PubMed] [Google Scholar]
- SILVERMAN M. P., LUNDGREN D. G. Studies on the chemoautotrophic iron bacterium Ferrobacillus ferrooxidans. I. An improved medium and a harvesting procedure for securing high cell yields. J Bacteriol. 1959 May;77(5):642–647. doi: 10.1128/jb.77.5.642-647.1959. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Shivvers D. W., Brock T. D. Oxidation of elemental sulfur by Sulfolobus acidocaldarius. J Bacteriol. 1973 May;114(2):706–710. doi: 10.1128/jb.114.2.706-710.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Singer P. C., Stumm W. Acidic mine drainage: the rate-determining step. Science. 1970 Feb 20;167(3921):1121–1123. doi: 10.1126/science.167.3921.1121. [DOI] [PubMed] [Google Scholar]
- Smith D. W., Fliermans C. B., Brock T. D. Technique for measuring 14 CO 2 uptake by soil microorganisms in situ. Appl Microbiol. 1972 Mar;23(3):595–600. doi: 10.1128/am.23.3.595-600.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Tuttle J. H., Dugan P. R., Macmillan C. B., Randles C. I. Microbial dissimilatory sulfur cycle in acid mine water. J Bacteriol. 1969 Feb;97(2):594–602. doi: 10.1128/jb.97.2.594-602.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Tuttle J. H., Randles C. I., Dugan P. R. Activity of microorganisms in acid mine water. I. Influence of acid water on aerobic heterotrophs of a normal stream. J Bacteriol. 1968 May;95(5):1495–1503. doi: 10.1128/jb.95.5.1495-1503.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]