Abstract
Hfr strains B4 and B8 transfer the Escherichia coli chromosome in opposite directions, each transferring lac+ as the last known marker. They were mated in concurrent crosses with the proA leu metE lys trp purE lac strain χ462. Analysis of the time of entry values for these markers showed that Hfr strain B8 transfers the whole chromosome more rapidly than does Hfr strain B4. In both crosses, the rate of transfer observed decelerates. If deceleration occurs as a function of the amount of chromosome transferred, the data are consistent with the markers examined being very accurately placed on the Taylor-Trotter map of the E. coli K-12 genome.
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Selected References
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- Berg C. M., Curtiss R., 3rd Transposition derivatives of an Hfr strain of Escherichia coli K-12. Genetics. 1967 Jul;56(3):503–525. doi: 10.1093/genetics/56.3.503. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Broda P. Modified map positions for lac and the pro markers in Escherichia coli K-12. J Bacteriol. 1974 Feb;117(2):741–746. doi: 10.1128/jb.117.2.741-746.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
- CLARK A. J., MARGULIES A. D. ISOLATION AND CHARACTERIZATION OF RECOMBINATION-DEFICIENT MUTANTS OF ESCHERICHIA COLI K12. Proc Natl Acad Sci U S A. 1965 Feb;53:451–459. doi: 10.1073/pnas.53.2.451. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Fulton C. Continuous chromosome transfer in Escherichia coli. Genetics. 1965 Jul;52(1):55–74. doi: 10.1093/genetics/52.1.55. [DOI] [PMC free article] [PubMed] [Google Scholar]
- JACOB F., WOLLMAN E. L. Genetic and physical determinations of chromosomal segments in Escherichia coli. Symp Soc Exp Biol. 1958;12:75–92. [PubMed] [Google Scholar]
- Low B. Inversion of transfer modes and sex factor-chromosome interactions in conjugation in Escherichia coli. J Bacteriol. 1967 Jan;93(1):98–106. doi: 10.1128/jb.93.1.98-106.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Masters M., Broda P. Evidence for the bidirectional replications of the Escherichia coli chromosome. Nat New Biol. 1971 Aug 4;232(31):137–140. doi: 10.1038/newbio232137a0. [DOI] [PubMed] [Google Scholar]
- TAYLOR A. L., THOMAN M. S. THE GENETIC MAP OF ESCHERICHIA COLI K-12. Genetics. 1964 Oct;50:659–677. doi: 10.1093/genetics/50.4.659. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Taylor A. L., Trotter C. D. Linkage map of Escherichia coli strain K-12. Bacteriol Rev. 1972 Dec;36(4):504–524. doi: 10.1128/br.36.4.504-524.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Wood T. H. Effects of temperature, agitation, and donor strain on chromosome transfer in Escherichia coli K-12. J Bacteriol. 1968 Dec;96(6):2077–2084. doi: 10.1128/jb.96.6.2077-2084.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]