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. 1974 Feb;117(2):775–782. doi: 10.1128/jb.117.2.775-782.1974

Transcription from the Complementary Deoxyribonucleic Acid Strands of Bacillus subtilis During Various Stages of Sporulation

Chikako Sumida-Yasumoto 1, Roy H Doi 1
PMCID: PMC285572  PMID: 4204440

Abstract

The messenger ribonucleic acid (mRNA) pattern of log-phase and sporulating cells of Bacillus subtilis has been analyzed by deoxyribonucleic acid (DNA)-RNA hybrid studies with the complementary-strand fragments of DNA. Approximately 80% of the mRNA of log-phase and sporulating cells from stages I, III, and IV hybridizes with the heavy DNA fragments, and 20% hybridizes with the light DNA fragments. Hybrid competition studies indicated that there was either a greatly reduced rate of transcription or a turn-off of some log-phase genes during the sporulation stages. However, a significant amount of log-phase gene transcription occurred even at late stages of sporulation. Similar studies indicate a significantly increased rate of transcription or a turn-on of sporulation phase genes during the latter stages of sproulation. There is a sequential increase in the amount of sporulation-specific transcription from both complementary-strand fragments of DNA. These results indicate that the RNA polymerase population in sporulating cells can transcribe both log-phase and sporulation-phase genes.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ARONSON A. I. CHARACTERIZATION OF MESSENGER RNA IN SPORULATING BACILLUS CEREUS. J Mol Biol. 1965 Mar;11:576–588. doi: 10.1016/s0022-2836(65)80012-2. [DOI] [PubMed] [Google Scholar]
  2. BALASSA G. RENOUVELLEMENT DE L'ACIDE RIBONUCL'EIQUE AU COURS DE LA SPORULATION DE BACILLUS SUBTILIS. Biochim Biophys Acta. 1963 Nov 22;76:410–416. doi: 10.1016/0006-3002(63)90060-x. [DOI] [PubMed] [Google Scholar]
  3. Bonamy C., Hirschbein L., Szulmajster J. Synthesis of ribosomal ribonucleic acid during sporulation of Bacillus subtilis. J Bacteriol. 1973 Mar;113(3):1296–1306. doi: 10.1128/jb.113.3.1296-1306.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. DOI R. H., IGARASHI R. T. GENETIC TRANSCRIPTION DURING MORPHOGENESIS. Proc Natl Acad Sci U S A. 1964 Sep;52:755–762. doi: 10.1073/pnas.52.3.755. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. DiCioccio R. A., Strauss N. Patterns of transcription in Bacillus subtilis during sporulation. J Mol Biol. 1973 Jun 25;77(2):325–336. doi: 10.1016/0022-2836(73)90338-0. [DOI] [PubMed] [Google Scholar]
  6. Doi R. H., Brown L. R., Rodgers G., Hsu Y. Bacillus subtilis mutant altered in spore morphology and in RNA polymerase activity. Proc Natl Acad Sci U S A. 1970 Jun;66(2):404–410. doi: 10.1073/pnas.66.2.404. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Hussey C., Losick R., Sonenshein A. L. Ribosomal RNA synthesis is turned off during sporulation of Bacillus subtilis. J Mol Biol. 1971 Apr 14;57(1):59–70. doi: 10.1016/0022-2836(71)90119-7. [DOI] [PubMed] [Google Scholar]
  8. Korch C. T., Doi R. H. Electron microscopy of the altered spore morphology of a ribonucleic acid polymerase mutant of Bacillus subtilis. J Bacteriol. 1971 Mar;105(3):1110–1118. doi: 10.1128/jb.105.3.1110-1118.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Leighton T. J. An RNA polymerase mutation causing temperature-sensitive sporulation in Bacillus subtilis. Proc Natl Acad Sci U S A. 1973 Apr;70(4):1179–1183. doi: 10.1073/pnas.70.4.1179. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Leighton T. J., Doi R. H. The stability of messenger ribonucleic acid during sporulation in Bacillus subtilis. J Biol Chem. 1971 May 25;246(10):3189–3195. [PubMed] [Google Scholar]
  11. Leighton T. J., Dor R. H., Warren R. A., Kelln R. A. The relationship of serine protease activity to RNA polymerase modification and sporulation in Bacillus subtilis. J Mol Biol. 1973 May 5;76(1):103–122. doi: 10.1016/0022-2836(73)90083-1. [DOI] [PubMed] [Google Scholar]
  12. Linn T. G., Greenleaf A. L., Shorenstein R. G., Losick R. Loss of the sigma activity of RNA polymerase of Bacillus subtilis during sporulation. Proc Natl Acad Sci U S A. 1973 Jun;70(6):1865–1869. doi: 10.1073/pnas.70.6.1865. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Losick R., Shorenstein R. G., Sonenshein A. L. Structural alteration of RNA polymerase during sporulation. Nature. 1970 Aug 29;227(5261):910–913. doi: 10.1038/227910a0. [DOI] [PubMed] [Google Scholar]
  14. Margulies L., Remeza V., Rudner R. Asymmetric template function of microbial deoxyribonucleic acids: transcription of messenger ribonucleic acid. J Bacteriol. 1971 Sep;107(3):610–617. doi: 10.1128/jb.107.3.610-617.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Margulies L., Remeza V., Rudner R. Asymmetric template function of microbial deoxyribonucleic acids: transcription of ribosomal and soluble ribonucleic acids. J Bacteriol. 1970 Sep;103(3):560–568. doi: 10.1128/jb.103.3.560-568.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. McConaughy B. L., Laird C. D., McCarthy B. J. Nucleic acid reassociation in formamide. Biochemistry. 1969 Aug;8(8):3289–3295. doi: 10.1021/bi00836a024. [DOI] [PubMed] [Google Scholar]
  17. Oishi M. The transcribing strands of bacillus subtilis DNA for ribosomal and transfer RNA. Proc Natl Acad Sci U S A. 1969 Jan;62(1):256–262. doi: 10.1073/pnas.62.1.256. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Rudner R., Ledoux M., Mazelis A. Distribution of pyrimidine oligonucleotides in strands L and H of Bacillus subtilis DNA. Proc Natl Acad Sci U S A. 1972 Sep;69(9):2745–2749. doi: 10.1073/pnas.69.9.2745. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. SZULMAJSTER J., CANFIELD R. E., BLICHARSKA J. [Action of actinomycin D on the sporulation of Bacillus subtilis]. C R Hebd Seances Acad Sci. 1963 Feb 25;256:2057–2060. [PubMed] [Google Scholar]
  20. Santo L., Leighton T. J., Doi R. H. Ultrastructural analysis of sporulation in a conditional serine protease mutant of Bacillus subtilis. J Bacteriol. 1972 Jul;111(1):248–253. doi: 10.1128/jb.111.1.248-253.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Sonenshein A. L., Losick R. RNA polymerase mutants blocked in sporulation. Nature. 1970 Aug 29;227(5261):906–909. doi: 10.1038/227906a0. [DOI] [PubMed] [Google Scholar]
  22. Tipper D. J., Pratt I. Cell wall polymers of Bacillus sphaericus 9602. II. Synthesis of the first enzyme unique to cortex synthesis during sporulation. J Bacteriol. 1970 Aug;103(2):305–317. doi: 10.1128/jb.103.2.305-317.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Weiss S. B., Hsu W. T., Foft J. W., Scherberg N. H. Transfer RNA coded by the T4 bacteriophage genome. Proc Natl Acad Sci U S A. 1968 Sep;61(1):114–121. doi: 10.1073/pnas.61.1.114. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Yamakawa T., Doi R. H. Preferential transcription of Bacillus subtilis light deoxyribonucleic acid strands during sporulation. J Bacteriol. 1971 May;106(2):305–310. doi: 10.1128/jb.106.2.305-310.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]

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