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. 2010 May;130(1):74–82. doi: 10.1111/j.1365-2567.2009.03214.x

Figure 1.

Figure 1

CCL26 messenger RNA (mRNA) is expressed in monocytic cells following stimulation with interleukin (IL)-4. (a and d) U937 cells, (b and e) monocyte-derived macrophages (MDMs), or (c and f) monocytes were cultured for 24 hr in medium alone (Con) or in mediim containing 10 ng/ml of IL-4, tumour necrosis factor-α (TNF-α), IL-1β or interferon-γ (IFN-γ). RNA was isolated from the cells using TRIzol. (a–c) The expression of mRNA for CCL26 and β-actin was evaluated using the reverse transcription–polymerase chain reaction (RT-PCR), and the PCR products were visualized by gel electrophoresis in the presence of ethidium bromide. Data are representative of results obtained from at least three independent experiments. (d–f) The expression of mRNA for CCL26 was evaluated using real-time PCR and data were normalized using 18S ribosomal RNA (rRNA) expression. Values for cytokine-stimulated cells were compared with values for control cells and data were then expressed as the mean ± standard error of the mean (SEM) of −delta delta Ct (−ddCt) from at least three independent experiments. **P<0·01 indicates statistical significance compared with the control.