Abstract
A procedure for the isolation of exo-β-N-acetylglucosaminidase mutants, by using a plate assay method incorporating a fluorescent substrate, has been developed. A mutant lacking exo-β-N-acetylglucosaminidase activity has been isolated and shown to grow, divide, autolyze, and sporulate as well as the parental strain.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Berkeley R. C., Brewer S. J., Ortiz J. M., Gillespie J. B. An exo- -N-acetylglucosaminidase from Bacillus subtilis B; characterization. Biochim Biophys Acta. 1973 May 5;309(1):157–168. doi: 10.1016/0005-2744(73)90327-6. [DOI] [PubMed] [Google Scholar]
- Fan D. P. Autolysin(s) of Bacillus subtilis as dechaining enzyme. J Bacteriol. 1970 Aug;103(2):494–499. doi: 10.1128/jb.103.2.494-499.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Fan D. P. Cell wall binding properties of the Bacillus subtilis autolysin(s). J Bacteriol. 1970 Aug;103(2):488–493. doi: 10.1128/jb.103.2.488-493.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Mauck J., Chan L., Glaser L. Turnover of the cell wall of Gram-positive bacteria. J Biol Chem. 1971 Mar 25;246(6):1820–1827. [PubMed] [Google Scholar]
- Ortiz J. M., Gillespie J. B., Berkeley R. C. An exo- -N-acetylglucosaminidase from Bacillus subtilis B; extraction and purification. Biochim Biophys Acta. 1972 Nov 10;289(1):174–186. doi: 10.1016/0005-2744(72)90120-9. [DOI] [PubMed] [Google Scholar]
- Robinson D., Price R. G., Dance N. Separation and properties of beta-galactosidase, beta-glucosidase, beta-glucuronidase and N-acetyl-beta-glucosaminidase from rat kidney. Biochem J. 1967 Feb;102(2):525–532. doi: 10.1042/bj1020525. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Rogers H. J. Bacterial growth and the cell envelope. Bacteriol Rev. 1970 Jun;34(2):194–214. doi: 10.1128/br.34.2.194-214.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Schaeffer P. Sporulation and the production of antibiotics, exoenzymes, and exotonins. Bacteriol Rev. 1969 Mar;33(1):48–71. doi: 10.1128/br.33.1.48-71.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Schwarz U., Asmus A., Frank H. Autolytic enzymes and cell division of Escherichia coli. J Mol Biol. 1969 May 14;41(3):419–429. doi: 10.1016/0022-2836(69)90285-x. [DOI] [PubMed] [Google Scholar]