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. 1974 Feb;117(2):917–919. doi: 10.1128/jb.117.2.917-919.1974

Mutant of Azotobacter vinelandii That Hyperproduces Nitrogenase Component II

Vinod K Shah 1, L C Davis 1, M Stieghorst 1, Winston J Brill 1
PMCID: PMC285593  PMID: 4204445

Abstract

A mutant strain of Azotobacter vinelandii that is unable to fix N2 produces high levels of nitrogenase component II. Activities of revertants from this mutant strain indicate that a single genetic lesion is responsible for both hyperproduction of component II and the inability to produce component I.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bulen W. A., LeComte J. R. The nitrogenase system from Azotobacter: two-enzyme requirement for N2 reduction, ATP-dependent H2 evolution, and ATP hydrolysis. Proc Natl Acad Sci U S A. 1966 Sep;56(3):979–986. doi: 10.1073/pnas.56.3.979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Fisher R. J., Brill W. J. Mutants of Azotobacter vinelandii unable to fix nitrogen. Biochim Biophys Acta. 1969 Jun 17;184(1):99–105. doi: 10.1016/0304-4165(69)90103-2. [DOI] [PubMed] [Google Scholar]
  3. PREER J. R., Jr A quantitative study of a technique of double diffusion in agar. J Immunol. 1956 Jul;77(1):52–60. [PubMed] [Google Scholar]
  4. Shah V. K., Brill W. J. Nitrogenase. IV. Simple method of purification to homogeneity of nitrogenase components from Azotobacter vinelandii. Biochim Biophys Acta. 1973 May 30;305(2):445–454. doi: 10.1016/0005-2728(73)90190-4. [DOI] [PubMed] [Google Scholar]
  5. Shah V. K., Davis I. C., Gordon J. K., Orme-Johnson W. H., Brill W. J. Nitrogenase. 3. Nitrogenaseless mutants of Azotobacter vinelandii: activities, cross-reactions and EPR spectra. Biochim Biophys Acta. 1973 Jan 18;292(1):246–255. doi: 10.1016/0005-2728(73)90269-7. [DOI] [PubMed] [Google Scholar]
  6. Shah V. K., Davis L. C., Brill W. J. Nitrogenase. I. Repression and derepression of the iron-molybdenum and iron proteins of nitrogenase in Azotobacter vinelandii. Biochim Biophys Acta. 1972 Feb 28;256(2):498–511. doi: 10.1016/0005-2728(72)90078-3. [DOI] [PubMed] [Google Scholar]
  7. Strandberg G. W., Wilson P. W. Formation of the nitrogen-fixing enzyme system in Azotobacter vinelandii. Can J Microbiol. 1968 Jan;14(1):25–31. doi: 10.1139/m68-005. [DOI] [PubMed] [Google Scholar]

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