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. Author manuscript; available in PMC: 2010 Apr 26.
Published in final edited form as: Proteomics. 2009 May;9(9):2468–2483. doi: 10.1002/pmic.200800613

Figure 7.

Figure 7

Apolipoprotein B in LDL. (Left) Western blot analysis (n = 3) revealed no significant changes in apolipoprotein B in LDL diseased pigs, although there was a wide variation in apolipoprotein B content in exercised pigs. The 500-kDa bands reacting with the anti-apolipoprotein B antibody are shown at the top of the bar graph. (Middle) LFQMS analysis (n = 4) revealed no significant changes in apolipoprotein B with any treatment groups. (Right) SRM (n = 4) revealed an increase in apolipoprotein B with exercise (DDX) compared to control and diabetic dyslipidemic groups (C and DD). However, there was substantial variation in apolipoprotein B level in exercised pigs using both Western blot and SRM analysis methods. The F-ratio was highest in the SRM experiment. Apolipoprotein B is too large to enter 2-D gels. * Denotes a significant difference compared to control (C) animals; †denotes a significant difference compared to diabetic dyslipidemic (DD) animals (p ≤0.01).