Skip to main content
NCBI home page
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1969 Jan;62(1):186–193. doi: 10.1073/pnas.62.1.186

PROPERTIES OF NUCLEAR RNA IN SEA URCHIN EMBRYOS*

Arthur I Aronson 1,2,†, Fred H Wilt 1,2
PMCID: PMC285972  PMID: 5253656

Abstract

Pulse-labeled RNA in early sea urchin embryos (64 cells to mesenchyme blastulase) is found almost exclusively associated with the nucleus and turns over very rapidly. The rate of labeling of the cytoplasm is much slower and is consistent with the transfer of 6 per cent of the nuclear RNA made every 15-20 minutes. Most of the pulse-labeled nuclear RNA exists in membrane-bound polyribosomes.

Full text

PDF
186

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Attardi G., Parnas H., Hwang M. I., Attardi B. Giant-size rapidly labeled nuclear ribonucleic acid and cytoplasmic messenger ribonucleic acid in immature duck erythrocytes. J Mol Biol. 1966 Sep;20(1):145–182. doi: 10.1016/0022-2836(66)90123-9. [DOI] [PubMed] [Google Scholar]
  2. Bach M. K., Johnson H. G. Polysomes on a nuclear membrane fraction as intermediates in the transfer of ribonucleic acid from the nucleus to the cytoplasm. Nature. 1966 Feb 26;209(5026):893–895. doi: 10.1038/209893a0. [DOI] [PubMed] [Google Scholar]
  3. Bonner J., Widholm J. Molecular complementarity between nuclear DNA and organ-specific chromosomal RNA. Proc Natl Acad Sci U S A. 1967 May;57(5):1379–1385. doi: 10.1073/pnas.57.5.1379. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Britten R. J., Kohne D. E. Repeated sequences in DNA. Hundreds of thousands of copies of DNA sequences have been incorporated into the genomes of higher organisms. Science. 1968 Aug 9;161(3841):529–540. doi: 10.1126/science.161.3841.529. [DOI] [PubMed] [Google Scholar]
  5. CERIOTTI G. A microchemical determination of desoxyribonucleic acid. J Biol Chem. 1952 Sep;198(1):297–303. [PubMed] [Google Scholar]
  6. Church R., McCarthy B. J. Changes in nuclear and cytoplasmic RNA in regenerating mouse liver. Proc Natl Acad Sci U S A. 1967 Oct;58(4):1548–1555. doi: 10.1073/pnas.58.4.1548. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. HINEGARDNER R. T. The isolation of nuclei from eggs and embryos of the sea urchin. J Cell Biol. 1962 Dec;15:503–508. doi: 10.1083/jcb.15.3.503. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. KATZ S., COMB D. G. A NEW METHOD FOR THE DETERMINATION OF THE BASE COMPOSITION OF RIBONUCLEIC ACID. J Biol Chem. 1963 Sep;238:3065–3067. [PubMed] [Google Scholar]
  9. McEwen C. R. Tables for estimating sedimentation through linear concentration gradients of sucrose solution. Anal Biochem. 1967 Jul;20(1):114–149. doi: 10.1016/0003-2697(67)90271-0. [DOI] [PubMed] [Google Scholar]
  10. Neuhard J., Randerath E., Randerath K. Ion-exchange thin-layer chromatography. 13. Resolution of complex nucleoside triphosphate mixtures. Anal Biochem. 1965 Nov;13(2):211–222. doi: 10.1016/0003-2697(65)90191-0. [DOI] [PubMed] [Google Scholar]
  11. Penman S., Vesco C., Penman M. Localization and kinetics of formation of nuclear heterodisperse RNA, cytoplasmic heterodisperse RNA and polyribosome-associated messenger RNA in HeLa cells. J Mol Biol. 1968 May 28;34(1):49–60. doi: 10.1016/0022-2836(68)90234-9. [DOI] [PubMed] [Google Scholar]
  12. SCHIMKE R. T. THE IMPORTANCE OF BOTH SYNTHESIS AND DEGRADATION IN THE CONTROL OF ARGINASE LEVELS IN RAT LIVER. J Biol Chem. 1964 Nov;239:3808–3817. [PubMed] [Google Scholar]
  13. Sadowski P. D., Howden J. A. Isolation of two distinct classes of polysomes from a nuclear fraction of rat liver. J Cell Biol. 1968 Apr;37(1):163–181. doi: 10.1083/jcb.37.1.163. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Scherrer K., Marcaud L., Zajdela F., London I. M., Gros F. Patterns of RNA metabolism in a differentiated cell: a rapidly labeled, unstable 60S RNA with messenger properties in duck erythroblasts. Proc Natl Acad Sci U S A. 1966 Nov;56(5):1571–1578. doi: 10.1073/pnas.56.5.1571. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Schimke R. T., Sweeney E. W., Berlin C. M. An analysis of the kinetics of rat liver tryptophan pyrrolase induction: the significance of both enzyme synthesis and degradation. Biochem Biophys Res Commun. 1964 Mar 26;15(3):214–219. doi: 10.1016/0006-291x(64)90148-2. [DOI] [PubMed] [Google Scholar]
  16. Warner J. R., Soeiro R., Birnboim H. C., Girard M., Darnell J. E. Rapidly labeled HeLa cell nuclear RNA. I. Identification by zone sedimentation of a heterogeneous fraction separate from ribosomal precursor RNA. J Mol Biol. 1966 Aug;19(2):349–361. doi: 10.1016/s0022-2836(66)80009-8. [DOI] [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES