Skip to main content
PMC home page
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1969 Jan;62(1):256–262. doi: 10.1073/pnas.62.1.256

THE TRANSCRIBING STRANDS OF BACILLUS SUBTILIS DNA FOR RIBOSOMAL AND TRANSFER RNA*

M Oishi 1
PMCID: PMC285981  PMID: 4976948

Abstract

H3-labeled purified 16S and 23S ribosomal RNA's and transfer RNA's were found to hybridize exclusively with the H strand of the separated complementary strands of B. subtilis DNA. These results indicate that these RNA's are transcribed in vivo from a strand with a composition similar to that from which messenger RNA's are copied and presumably by the same mechanism.

Full text

PDF

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Dubnau D., Goldthwaite C., Smith I., Marmur J. Genetic mapping in Bacillus subtilis. J Mol Biol. 1967 Jul 14;27(1):163–185. doi: 10.1016/0022-2836(67)90358-0. [DOI] [PubMed] [Google Scholar]
  2. Dubnau D., Smith I., Marmur J. Gene conservation in Bacillus species. II. The location of genes concerned with the synthesis of ribosomal components and soluble RNA. Proc Natl Acad Sci U S A. 1965 Sep;54(3):724–730. doi: 10.1073/pnas.54.3.724. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Dubnau D., Smith I., Morell P., Marmur J. Gene conservation in Bacillus species. I. Conserved genetic and nucleic acid base sequence homologies. Proc Natl Acad Sci U S A. 1965 Aug;54(2):491–498. doi: 10.1073/pnas.54.2.491. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Gillespie D., Spiegelman S. A quantitative assay for DNA-RNA hybrids with DNA immobilized on a membrane. J Mol Biol. 1965 Jul;12(3):829–842. doi: 10.1016/s0022-2836(65)80331-x. [DOI] [PubMed] [Google Scholar]
  5. Guha A., Szybalski W. Fractionation of the complementary strands of coliphage T4 DNA based on the asymmetric distribution of the poly U and poly U,G binding sites. Virology. 1968 Apr;34(4):608–616. doi: 10.1016/0042-6822(68)90082-2. [DOI] [PubMed] [Google Scholar]
  6. Hogness D. S., Doerfler W., Egan J. B., Black L. W. The position and orientation of genes in lambda and lambda dg DNA. Cold Spring Harb Symp Quant Biol. 1966;31:129–138. doi: 10.1101/sqb.1966.031.01.020. [DOI] [PubMed] [Google Scholar]
  7. Karkas J. D., Rudner R., Chargaff E. Seapration of B. subtilis DNA into complementary strands. II. Template functions and composition as determined by transcription with RNA polymerase. Proc Natl Acad Sci U S A. 1968 Jul;60(3):915–920. doi: 10.1073/pnas.60.3.915. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Kubinski H., Opara-Kubinska Z., Szybalski W. Patterns of interaction between polyribonucleotides and individual DNA strands derived from several vertebrates, bacteria and bacteriophages. J Mol Biol. 1966 Sep;20(2):313–329. doi: 10.1016/0022-2836(66)90067-2. [DOI] [PubMed] [Google Scholar]
  9. MANDELL J. D., HERSHEY A. D. A fractionating column for analysis of nucleic acids. Anal Biochem. 1960 Jun;1:66–77. doi: 10.1016/0003-2697(60)90020-8. [DOI] [PubMed] [Google Scholar]
  10. MARGOLIN P. BIPOLARITY OF INFORMATION TRANSFER FROM THE SALMONELLA TYPHIMURIUM CHROMOSOME. Science. 1965 Mar 19;147(3664):1456–1458. doi: 10.1126/science.147.3664.1456. [DOI] [PubMed] [Google Scholar]
  11. MIURA K. I. The nucleotide composition of ribonucleic acids of soluble and particle fractions in several species of bacteria. Biochim Biophys Acta. 1962 Jan 22;55:62–70. doi: 10.1016/0006-3002(62)90931-9. [DOI] [PubMed] [Google Scholar]
  12. NYGAARD A. P., HALL B. D. A method for the detection of RNA-DNA complexes. Biochem Biophys Res Commun. 1963 Jul 18;12:98–104. doi: 10.1016/0006-291x(63)90242-0. [DOI] [PubMed] [Google Scholar]
  13. OISHI M., YOSHIKAWA H., SUEOKA N. SYNCHRONOUS AND DICHOTOMOUS REPLICATIONS OF THE BACILLUS SUBTILIS CHROMOSOME DURING SPORE GERMINATION. Nature. 1964 Dec 12;204:1069–1073. doi: 10.1038/2041069a0. [DOI] [PubMed] [Google Scholar]
  14. OPARA-KUBINSKA Z., KUBINSKI H., SZYBALSKI W. INTERACTION BETWEEN DENATURED DNA, POLYRIBONUCLEOTIDES, AND RIBOSOMAL RNA: ATTEMPTS AT PREPARATIVE SEPARATION OF THE COMPLEMENTARY DNA STRANDS. Proc Natl Acad Sci U S A. 1964 Oct;52:923–930. doi: 10.1073/pnas.52.4.923. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Oishi M., Oishi A., Sueoka N. Location of genetic loci of soluble RNA on Bacillus subtilis chromosome. Proc Natl Acad Sci U S A. 1966 May;55(5):1095–1103. doi: 10.1073/pnas.55.5.1095. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Oishi M., Sueoka N. Location of genetic loci of ribosomal RNA on Bacillus subtilis chromosome. Proc Natl Acad Sci U S A. 1965 Aug;54(2):483–491. doi: 10.1073/pnas.54.2.483. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Rudner R., Karkas J. D., Chargaff E. Separation of B. subtilis DNA into complementary strands, I. Biological properties. Proc Natl Acad Sci U S A. 1968 Jun;60(2):630–635. doi: 10.1073/pnas.60.2.630. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. SAITO H., MIURA K. I. PREPARATION OF TRANSFORMING DEOXYRIBONUCLEIC ACID BY PHENOL TREATMENT. Biochim Biophys Acta. 1963 Aug 20;72:619–629. [PubMed] [Google Scholar]
  19. STUDIER F. W. SEDIMENTATION STUDIES OF THE SIZE AND SHAPE OF DNA. J Mol Biol. 1965 Feb;11:373–390. doi: 10.1016/s0022-2836(65)80064-x. [DOI] [PubMed] [Google Scholar]
  20. SUEOKA N., CHENG T. Y. Fractionation of nucleic acids with the methylated albumin column. J Mol Biol. 1962 Mar;4:161–172. doi: 10.1016/s0022-2836(62)80048-5. [DOI] [PubMed] [Google Scholar]
  21. Sanderson K. E. Information transfer in Salmonella typhimurium. Proc Natl Acad Sci U S A. 1965 Jun;53(6):1335–1340. doi: 10.1073/pnas.53.6.1335. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Smith I., Dubnau D., Morrell P., Marmur J. Chromosomal location of DNA base sequences complementary to transfer RNA and to 5 s, 16 s and 23 s ribosomal RNA in Bacillus subtilis. J Mol Biol. 1968 Apr 14;33(1):123–140. doi: 10.1016/0022-2836(68)90285-4. [DOI] [PubMed] [Google Scholar]
  23. Streisinger G., Emrich J., Okada Y., Tsugita A., Inouye M. Direction of translation of the lysozyme gene of bacteriophage T4 relative to the linkage map. J Mol Biol. 1968 Feb 14;31(3):607–612. doi: 10.1016/0022-2836(68)90431-2. [DOI] [PubMed] [Google Scholar]
  24. Szybalski W., Kubinski H., Sheldrick P. Pyrimidine clusters on the transcribing strand of DNA and their possible role in the initiation of RNA synthesis. Cold Spring Harb Symp Quant Biol. 1966;31:123–127. doi: 10.1101/sqb.1966.031.01.019. [DOI] [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES