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. Author manuscript; available in PMC: 2011 May 5.
Published in final edited form as: Neuroscience. 2010 Feb 6;167(2):554–566. doi: 10.1016/j.neuroscience.2010.01.043

Table 5.

Real-time RT-PCR results compared with microarray data (expressed as percent of control.

Hypothalamus 4 d Ratio of expression
following 4 d of
morphine
treatment
compared to
placebo treatment		 Ratio of expression
following 4 d of
morphine
treatment
compared to
placebo treatment
Gene GenBank # Microarray Real-time RT-PCR
Unknown (RIKEN
cDNA 5730513H21
gene)		 AK017769 0.67 0.42 ± 0.2
Unknown (RIKEN
cDNA 3110001K13
gene)		 AK013955 0.44 0.78 ± 0.01
AgRP NM_007427 2.4 3.4 ± 0.01
Npy NM_023456 2.6 4.3 ± 0.1
proSAAS NM_013892 0.94 1.1 ± 0.1
Pituitary 4 d
Gene GenBank Microarray Real-time RT-PCR
ACP homolog 0.38 0.64 ± 0.02
(RIKEN cDNA
2610003B19 gene)		 AK008788
AgRP NM_007427 1.5 3.6 ± 0.2
Npy NM_023456 1.5 4.3 ± 0.2
proSAAS NM_013892 1.3 1.2 ± 0.04

ACP Acyl Carrier Protein, AgRP agouti-related protein, Npy neuropeptide Y. The table lists the genes that were significantly regulated (p≤ 0.05). Data is expressed as mean ± SEM. RT-PCR results are from 2 separate pools of mRNA each from 3 animals.