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Fibroblasts align to the 100-µm ridge topography. (A) 100-µm ridge topography in silicone. (B) 100-µm ridge topography highlighted with white lines. (C) The cells along the surface of this feature are unable to reach down from this feature and as such grow along it. (D) Fibroblasts send filopodia along the edge of this feature (highlighted by the white arrow within this figure), searching along is edge, a classic example of contact guidance. Actin fibers (red) and vimentin (green).
