TABLE 4.
Ribozyme | Nucleophile | kc | |
---|---|---|---|
(min-1) | Relative | ||
WT | AUCG | 0.48 | (1.0) |
AUCI | 0.13 | 3.7 | |
A261H | AUCG | 0.023 | 21 |
AUCI | 0.065 | 7.4 |
Values of were > 1 for both the WT and A261H ribozymes (Table 3). Thus, both ribozymes reacted from the docked conformation.