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. 1970 May;66(1):160–167. doi: 10.1073/pnas.66.1.160

Regulation of Axon Formation by Clonal Lines of a Neural Tumor

N W Seeds 1,*, A G Gilman 1, T Amano 1, M W Nirenberg 1
PMCID: PMC286102  PMID: 5273894

Abstract

Clonal lines of neuroblastoma cells were found to extend or retract axons depending upon the concentration of serum. Neurite extension was not inhibited by cycloheximide but was sensitive to colchicine or vinblastine, suggesting that neurite formation is dependent upon the assembly of microtubules or neurofilaments from preformed protein subunits.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Augusti-Tocco G., Sato G. Establishment of functional clonal lines of neurons from mouse neuroblastoma. Proc Natl Acad Sci U S A. 1969 Sep;64(1):311–315. doi: 10.1073/pnas.64.1.311. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. LIEBERMAN I., OVE P. A protein growth factor for mammalian cells in culture. J Biol Chem. 1958 Sep;233(3):637–642. [PubMed] [Google Scholar]
  3. Marantz R., Ventilla M., Shelanski M. Vinblastine-induced precipitation of microtubule protein. Science. 1969 Aug 1;165(3892):498–499. doi: 10.1126/science.165.3892.498. [DOI] [PubMed] [Google Scholar]
  4. Nelson P., Ruffner W., Nirenberg M. Neuronal tumor cells with excitable membranes grown in vitro. Proc Natl Acad Sci U S A. 1969 Nov;64(3):1004–1010. doi: 10.1073/pnas.64.3.1004. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Olmsted J. B., Carlson K., Klebe R., Ruddle F., Rosenbaum J. Isolation of microtubule protein from cultured mouse neuroblastoma cells. Proc Natl Acad Sci U S A. 1970 Jan;65(1):129–136. doi: 10.1073/pnas.65.1.129. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Puck T. T., Waldren C. A., Jones C. Mammalian cell growth proteins. I. Growth stimulation of fetuin. Proc Natl Acad Sci U S A. 1968 Jan;59(1):192–199. doi: 10.1073/pnas.59.1.192. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Rosenbaum J. L., Moulder J. E., Ringo D. L. Flagellar elongation and shortening in Chlamydomonas. The use of cycloheximide and colchicine to study the synthesis and assembly of flagellar proteins. J Cell Biol. 1969 May;41(2):600–619. doi: 10.1083/jcb.41.2.600. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Schubert D., Humphreys S., Baroni C., Cohn M. In vitro differentiation of a mouse neuroblastoma. Proc Natl Acad Sci U S A. 1969 Sep;64(1):316–323. doi: 10.1073/pnas.64.1.316. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Todaro G. J., Lazar G. K., Green H. The initiation of cell division in a contact-inhibited mammalian cell line. J Cell Physiol. 1965 Dec;66(3):325–333. doi: 10.1002/jcp.1030660310. [DOI] [PubMed] [Google Scholar]
  10. Weisenberg R. C., Borisy G. G., Taylor E. W. The colchicine-binding protein of mammalian brain and its relation to microtubules. Biochemistry. 1968 Dec;7(12):4466–4479. doi: 10.1021/bi00852a043. [DOI] [PubMed] [Google Scholar]

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