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. 1970 May;66(1):197–203. doi: 10.1073/pnas.66.1.197

Complementarity of RNA Produced by Enzymic Transcription of Native and Denatured B. subtilis DNA*

Era Cassuto 1,†, Marshall Stein 1, Erwin Chargaff 1
PMCID: PMC286107  PMID: 4991519

Abstract

This paper describes the preparation and some of the properties of the RNA specimens synthesized with the aid of the RNA polymerase of E. coli by transcription of the following DNA templates: (a) undenatured B. subtilis DNA (yielding N-RNA); (b) separated strand fractions L and H isolated by chromatography of the denatured DNA on methylated albumin (yielding L-RNA and H-RNA, respectively). The study of the hybridization behavior of the various RNA products showed that N-RNA, though able to form hybrids with either strand, hybridized with H-DNA to twice as great an extent as with L-DNA. The transcripts of the separated L and H fractions exhibited complete specificity with respect to complexing: L-RNA hybridized only with L-DNA, H-RNA only with H-DNA.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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