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Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1970 May;66(1):204–210. doi: 10.1073/pnas.66.1.204

Conditions Determining Initiation of DNA Synthesis in 3T3 Cells*

R Dulbecco 1,, M G P Stoker 1,
PMCID: PMC286108  PMID: 5273897

Abstract

Experiments were designed to discriminate between inhibition of growth due to contacts or exhaustion of serum factors. The cell layer was wounded and the migrating cells were followed by time-lapse cinematography; DNA synthesis in the same cells was recognized by means of 3H-thymidine labeling and radioautography. In this way, the complete history of individual cells migrating to the wound could be described. The results show that topographical relationships between cells play an important role in controlling initiation of DNA synthesis. It is still unclear whether initiation is promoted by release from contacts or by the increased ability of the cells to utilize serum factors because of their changes in shapes and activities.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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