Abstract
BALB/c mouse embryo cells maintained in tissue culture on a schedule of rapid transfer at high cell density develop into tumorigenic lines that have lost contact inhibition of cell division. After several months in culture, certain of these lines begin to release mouse leukemia virus. This virus has properties indistinguishable from those of virus found in adult BALB/c mice. The evidence presented here demonstrates that the murine leukemia virus genome must be present in the original embryo cultures. The possibility that the genetic information for making murine leukemia virus is present in a repressed form in every mouse embryo cell is discussed.
Full text
PDF







Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Aaronson S. A., Todaro G. J. Basis for the acquisition of malignant potential by mouse cells cultivated in vitro. Science. 1968 Nov 29;162(3857):1024–1026. doi: 10.1126/science.162.3857.1024. [DOI] [PubMed] [Google Scholar]
- Aaronson S. A., Todaro G. J. Development of 3T3-like lines from Balb-c mouse embryo cultures: transformation susceptibility to SV40. J Cell Physiol. 1968 Oct;72(2):141–148. doi: 10.1002/jcp.1040720208. [DOI] [PubMed] [Google Scholar]
- GROSS L. Influence of ether, in vitro, on pathogenic properties of mouse leukemia extracts. Acta Haematol. 1956 May;15(5):273–277. doi: 10.1159/000205096. [DOI] [PubMed] [Google Scholar]
- Hartley J. W., Rowe W. P., Capps W. I., Huebner R. J. Complement fixation and tissue culture assays for mouse leukemia viruses. Proc Natl Acad Sci U S A. 1965 May;53(5):931–938. doi: 10.1073/pnas.53.5.931. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hartley J. W., Rowe W. P., Capps W. I., Huebner R. J. Isolation of naturally occurring viruses of the murine leukemia virus group in tissue culture. J Virol. 1969 Feb;3(2):126–132. doi: 10.1128/jvi.3.2.126-132.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Huebner R. J. The murine leukemia-sarcoma virus complex. Proc Natl Acad Sci U S A. 1967 Sep;58(3):835–842. doi: 10.1073/pnas.58.3.835. [DOI] [PMC free article] [PubMed] [Google Scholar]
- LEVINE M., BORTHWICK M. THE ACTION OF STREPTONIGRIN ON BACTERIAL DNA METABOLISM AND ON INDUCTION OF PHAGE PRODUCTION IN LYSOGENIC BACTERIA. Virology. 1963 Dec;21:568–574. doi: 10.1016/0042-6822(63)90228-9. [DOI] [PubMed] [Google Scholar]
- LIEBERMAN M., HARAN-GHERA N., KAPLAN H. S. POTENTIATION OF VIRUS LEUKAEMOGENESIS IN C57BL MICE BY X-IRRADIATION OR URETHANE. Nature. 1964 Jul 25;203:420–422. doi: 10.1038/203420b0. [DOI] [PubMed] [Google Scholar]
- Nakata Y., Bader J. P. Transformation by murine sarcoma virus: fixation (deoxyribonucleic acid synthesis) and development. J Virol. 1968 Nov;2(11):1255–1261. doi: 10.1128/jvi.2.11.1255-1261.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Ptashne M. ISOLATION OF THE lambda PHAGE REPRESSOR. Proc Natl Acad Sci U S A. 1967 Feb;57(2):306–313. doi: 10.1073/pnas.57.2.306. [DOI] [PMC free article] [PubMed] [Google Scholar]
- TODARO G. J., GREEN H. Quantitative studies of the growth of mouse embryo cells in culture and their development into established lines. J Cell Biol. 1963 May;17:299–313. doi: 10.1083/jcb.17.2.299. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Todaro G. J., Lazar G. K., Green H. The initiation of cell division in a contact-inhibited mammalian cell line. J Cell Physiol. 1965 Dec;66(3):325–333. doi: 10.1002/jcp.1030660310. [DOI] [PubMed] [Google Scholar]