Abstract
Sorting-out and other morphogenetic rearrangements of embryonic cells have been attributed to the spontaneous movements of mutually adhesive cells toward configurations of minimal adhesive free energy. Testing this hypothesis requires measuring those particular adhesive properties that would determine such equilibrium configurations.
We have devised a modification of the sessile drop method for making the required measurements.
This communication is a preliminary report of experiments demonstrating that intercellular adhesiveness is reflected in the equilibrium shapes of centrifuged cell aggregates.
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Selected References
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- EASTY G. C., EASTY D. M., AMBROSE E. J. Studies of cellular adhesiveness. Exp Cell Res. 1960 Apr;19:539–548. doi: 10.1016/0014-4827(60)90062-8. [DOI] [PubMed] [Google Scholar]
- Roth S. A., Weston J. A. The measurement of intercellular adhesion. Proc Natl Acad Sci U S A. 1967 Sep;58(3):974–980. doi: 10.1073/pnas.58.3.974. [DOI] [PMC free article] [PubMed] [Google Scholar]
- STEINBERG M. S. On the mechanism of tissue reconstruction by dissociated cells. I. Population kinetics, differential adhesiveness. and the absence of directed migration. Proc Natl Acad Sci U S A. 1962 Sep 15;48:1577–1582. doi: 10.1073/pnas.48.9.1577. [DOI] [PMC free article] [PubMed] [Google Scholar]
- STEINBERG M. S. Reconstruction of tissues by dissociated cells. Some morphogenetic tissue movements and the sorting out of embryonic cells may have a common explanation. Science. 1963 Aug 2;141(3579):401–408. doi: 10.1126/science.141.3579.401. [DOI] [PubMed] [Google Scholar]
- Steinberg M. S. ON THE MECHANISM OF TISSUE RECONSTRUCTION BY DISSOCIATED CELLS, III. FREE ENERGY RELATIONS AND THE REORGANIZATION OF FUSED, HETERONOMIC TISSUE FRAGMENTS. Proc Natl Acad Sci U S A. 1962 Oct;48(10):1769–1776. doi: 10.1073/pnas.48.10.1769. [DOI] [PMC free article] [PubMed] [Google Scholar]