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. Author manuscript; available in PMC: 2010 Jul 10.
Published in final edited form as: Cell. 2009 Jul 10;138(1):78–89. doi: 10.1016/j.cell.2009.06.029

FIGURE 2. Human SLX4/MUS312 interacts with SLX1 and XPF.

FIGURE 2

A- Y2H analysis of interactions between full length SLX4 fused to the GAL4-AD and full length or truncated SLX1 fused to the GAL4-DBD.

B- Y2H analysis of interactions between full length SLX1 fused to GAL4-DBD and various SLX4 truncations fused to GAL4-AD.

C- Immunoprecipitation of the indicated FLAG-tagged SLX1 proteins overproduced in HEK cells along with the indicated GFP-tagged SLX4 proteins. Western blot shows FLAG-tagged SLX1, GFP-tagged SLX4 and endogenous XPF in total cell lysates and FLAG-eluates. The amount of total cell lysate loaded per lane is 10% of the input, relative to the amount loaded of the corresponding FLAG-eluate.

D- Y2H analysis of interactions between SLX4 and SLX1 fused to GAL4-AD and XPF and ERCC1 fused to GAL4-DBD.

E- Y2H analysis of interaction between XPF fused to GAL4-DBD and the indicated SLX4 fragments fused to GAL4-AD.