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. 1969 Sep;64(1):283–289. doi: 10.1073/pnas.64.1.283

FRACTIONATION OF PHYTOHEMAGGLUTININ, I. PURIFICATION OF THE RNA AND DNA SYNTHESIS-STIMULATING SUBSTANCES AND EVIDENCE THAT THEY ARE NOT PROTEINS*

M L Goldberg 1,2, W Rosenau 1,2, G C Burke 1,2
PMCID: PMC286159  PMID: 5263012

Abstract

We have separated the RNA synthesis-stimulating activity and the DNA synthesis-stimulating activity of extracts of Phaseolus vulgaris (phytohemagglutinin) from the erythroagglutinins, leukoagglutinins, and cytotoxic factors. We have shown that the substances stimulating the synthesis of nucleic acids are probably not proteins, since they are not significantly affected by rigorous deproteinization or treatment by trypsin and pronase. Likewise, they do not appear to be nucleic acids, since they are not precipitated by perchloric acid and resist RNase, DNase, and phosphodiesterases. They are probably not carbohydrates, since 98 per cent of the carbohydrate can be removed from a preparation with only a moderate loss in activity. The RNA and DNA synthesis-stimulating activities are probably associated with separate molecules, since they are destroyed by periodate at different rates.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BARKHAN P., BALLAS A. PHYTOHAEMAGGLUTININ: SEPARATION OF HAEMAGGLUTINATING AND MITOGENIC PRINCIPLES. Nature. 1963 Oct 12;200:141–142. doi: 10.1038/200141a0. [DOI] [PubMed] [Google Scholar]
  2. Bennett T. P. Membrane filtration for determining protein in the presence of interfering substances. Nature. 1967 Mar 18;213(5081):1131–1132. doi: 10.1038/2131131a0. [DOI] [PubMed] [Google Scholar]
  3. COULSON A. S., CHALMERS D. G. SEPARATION OF VIABLE LYMPHOCYTES FROM HUMAN BLOOD. Lancet. 1964 Feb 29;1(7331):468–469. doi: 10.1016/s0140-6736(64)90799-8. [DOI] [PubMed] [Google Scholar]
  4. DISCHE Z. New color reactions for determination of sugars in polysaccharides. Methods Biochem Anal. 1955;2:313–358. doi: 10.1002/9780470110188.ch11. [DOI] [PubMed] [Google Scholar]
  5. HIRSCHHORN K., BACH F., KOLODNY R. L., FIRSCHEIN I. L., HASHEM N. IMMUNE RESPONSE AND MITOSIS OF HUMAN PERIPHERAL BLOOD LYMPHOCYTES IN VITRO. Science. 1963 Nov 29;142(3596):1185–1187. doi: 10.1126/science.142.3596.1185. [DOI] [PubMed] [Google Scholar]
  6. Hedeskov C. J. Early effects of phytohaemagglutinin on glucose metabolism of normal human lymphocytes. Biochem J. 1968 Nov;110(2):373–380. doi: 10.1042/bj1100373. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. JANOWSKY D. S., ROSENAU W., MOON H. D. ISOLATION OF IMMUNOLOGICALLY COMPETENT LYMPHOCYTES FROM SENSITIZED MOUSE SPLEENS. Proc Soc Exp Biol Med. 1964 Jan;115:77–79. doi: 10.3181/00379727-115-28835. [DOI] [PubMed] [Google Scholar]
  8. RIGAS D. A., JOHNSON E. A. STUDIES ON THE PHYTOHEMAGGLUTININ OF PHASEOLUS VULGARIS AND ITS MITOGENICITY. Ann N Y Acad Sci. 1964 Feb 28;113:800–818. doi: 10.1111/j.1749-6632.1964.tb40705.x. [DOI] [PubMed] [Google Scholar]
  9. Rigas D. A., Head C. The dissociation of phytohemagglutinin of Phaseolus vulgaris by 8.0 M urea and the separation of the mitogenic from the erythroagglutinating activity. Biochem Biophys Res Commun. 1969 Mar 10;34(5):633–639. doi: 10.1016/0006-291x(69)90785-2. [DOI] [PubMed] [Google Scholar]
  10. SEVER J. L. Application of a microtechnique to viral serological investigations. J Immunol. 1962 Mar;88:320–329. [PubMed] [Google Scholar]

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