Abstract
3′,5′-Cyclic nucleotides are acrasins for the cellular slime mold, Dictyostelium discoideum; they have chemotactic activity on the myxamoebae at very low concentrations. However, not all cyclic nucleotides can evoke the adhesiveness necessary for aggregate formation. 2′,3′-Cyclic nucleotides and dibutyrylcyclic adenosine monophosphate are not acrasins, though they enhance the rate of differentiation and morphogenesis. The addition of 3′,5′-cyclic nucleotides to agar test plates stimulates the rate of morphogenesis. While the stimulatory effect of cyclic nucleotides is concentration-dependent, it is fairly uniform between pH 5 and 7, with an optimum at pH 6. The acrasins may be metabolized by extracellular phosphodiesterase to 5′-nucelotides which may then stimulate differentiation and morphogenesis.
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