Skip to main content
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1970 Jan;65(1):81–87. doi: 10.1073/pnas.65.1.81

Trans-Species Rescue of Defective Genomes of Murine Sarcoma Virus from Hamster Tumor Cells with Helper Feline Leukemia Virus*

Padman S Sarma 1,2,, T Log 1,2,, Robert J Huebner 1,2,
PMCID: PMC286194  PMID: 4313195

Abstract

This report describes a trans-species rescue of defective MSV genome with helper leukemia virus derived from cats. This rescue was achieved by in vitro co-cultivation of hamster tumor cells with feline embryo cells in the presence of helper feline leukemia virus (FeLV), or by inoculation of tumor cells into FeLV-infected newborn cats.

The rescued focus-forming viruses produced foci in feline embryo cultures but not in cultures of mouse, rat, and hamster species. One isolate was tested and found to induce sarcoma in a kitten. Antigenic and viral interference studies indicated that the focus-forming virus has the viral envelope of FeLV. Virus stocks consisted of a mixture of focus-forming particles and a 1000-fold excess of helper FeLV. Virus assay pattern in feline embryo cultures with or without added helper FeLV indicated that this helper virus is required for the transformation of feline cells.

Full text

PDF
81

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bassin R. H., Simons P. J., Chesterman F. C., Harvey J. J. Murine sarcoma virus (Harvey): characteristics of focus formation in mouse embryo cell cultures, and virus production by hamster tumor cells. Int J Cancer. 1968 Mar 15;3(2):265–272. doi: 10.1002/ijc.2910030212. [DOI] [PubMed] [Google Scholar]
  2. Geering G., Hardy W. D., Jr, Old L. J., de Harven E., Brodey R. S. Shared group-specific antigen of murine and feline leukemia viruses. Virology. 1968 Dec;36(4):678–680. doi: 10.1016/0042-6822(68)90199-2. [DOI] [PubMed] [Google Scholar]
  3. Gregoriades A., Old L. J. Isolation and some characteristics of a group-specific antigen of the murine leukemia viruses. Virology. 1969 Feb;37(2):189–202. doi: 10.1016/0042-6822(69)90198-6. [DOI] [PubMed] [Google Scholar]
  4. HANAFUSA H. ANALYSIS OF THE DEFECTIVENESS OF ROUS SARCOMA VIRUS. 3. DETERMINING INFLUENCE OF A NEW HELPER VIRUS ON THE HOST RANGE AND SUSCEPTIBILITY TO INTERFERENCE OF RSV. Virology. 1965 Feb;25:248–255. doi: 10.1016/0042-6822(65)90203-5. [DOI] [PubMed] [Google Scholar]
  5. HANAFUSA H., HANAFUSA T., RUBIN H. The defectiveness of Rous sarcoma virus. Proc Natl Acad Sci U S A. 1963 Apr;49:572–580. doi: 10.1073/pnas.49.4.572. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. HARVEY J. J. AN UNIDENTIFIED VIRUS WHICH CAUSES THE RAPID PRODUCTION OF TUMOURS IN MICE. Nature. 1964 Dec 12;204:1104–1105. doi: 10.1038/2041104b0. [DOI] [PubMed] [Google Scholar]
  7. HUEBNER R. J., ARMSTRONG D., OKUYAN M., SARMA P. S., TURNER H. C. SPECIFIC COMPLEMENT-FIXING VIRAL ANTIGENS IN HAMSTER AND GUINEA PIG TUMORS INDUCED BY THE SCHMIDT-RUPPIN STRAIN OF AVIAN SARCOMA. Proc Natl Acad Sci U S A. 1964 May;51:742–750. doi: 10.1073/pnas.51.5.742. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Hanafusa H., Hanafusa T. Determining factor in the capacity of Rous sarcoma virus to induce tumors in mammals. Proc Natl Acad Sci U S A. 1966 Mar;55(3):532–538. doi: 10.1073/pnas.55.3.532. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Hanafusa H., Hanafusa T. Further studies on RSV production form transformed cells. Virology. 1968 Apr;34(4):630–636. doi: 10.1016/0042-6822(68)90084-6. [DOI] [PubMed] [Google Scholar]
  10. Hartley J. W., Rowe W. P., Capps W. I., Huebner R. J. Isolation of naturally occurring viruses of the murine leukemia virus group in tissue culture. J Virol. 1969 Feb;3(2):126–132. doi: 10.1128/jvi.3.2.126-132.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Hartley J. W., Rowe W. P. Production of altered cell foci in tissue culture by defective Moloney sarcoma virus particles. Proc Natl Acad Sci U S A. 1966 Apr;55(4):780–786. doi: 10.1073/pnas.55.4.780. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Huebner R. J., Hartley J. W., Rowe W. P., Lane W. T., Capps W. I. Rescue of the defective genome of Moloney sarcoma virus from a noninfectious hamster tumor and the production of pseudotype sarcoma viruses with various murine leukemia viruses. Proc Natl Acad Sci U S A. 1966 Oct;56(4):1164–1169. doi: 10.1073/pnas.56.4.1164. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. JARRETT W. F., CRAWFORD E. M., MARTIN W. B., DAVIE F. A VIRUS-LIKE PARTICLE ASSOCIATED WITH LEUKEMIA (LYMPHOSARCOMA). Nature. 1964 May 9;202:567–569. doi: 10.1038/202567a0. [DOI] [PubMed] [Google Scholar]
  14. JARRETT W. F., MARTIN W. B., CRIGHTON G. W., DALTON R. G., STEWART M. F. TRANSMISSION EXPERIMENTS WITH LEUKEMIA (LYMPHOSARCOMA). Nature. 1964 May 9;202:566–567. doi: 10.1038/202566a0. [DOI] [PubMed] [Google Scholar]
  15. Kawakami T. G., Theilen G. H., Dungworth D. L., Munn R. J., Beall S. G. "C"-type viral particles in plasma of cats with feline leukemia. Science. 1967 Nov;158(3804):1049–1050. doi: 10.1126/science.158.3804.1049. [DOI] [PubMed] [Google Scholar]
  16. Klement V., Hartley J. W., Rowe W. P., Huebner R. J. Recovery of a hamster-specific, focus-forming, and sarcomagenic virus from a "noninfectious" hamster tumor induced by the Kirsten Mouse sarcoma virus. J Natl Cancer Inst. 1969 Oct;43(4):925–934. [PubMed] [Google Scholar]
  17. Laird H. M., Jarrett O., Crighton G. W., Jarrett W. F., Hay D. Replication of leukemogenic-type virus in cats inoculated with feline lymphosarcoma extracts. J Natl Cancer Inst. 1968 Oct;41(4):879–893. [PubMed] [Google Scholar]
  18. Rhim J. S., Huebner R. J., Ting R. C. Trasformation of hamster embryo cells in vitro by Ruscher leukemia virus. J Natl Cancer Inst. 1969 Jun;42(6):1053–1060. [PubMed] [Google Scholar]
  19. Rickard C. G., Barr L. M., Noronha F., Dougherty E., 3rd, Post J. E. C-type virus particles in spontaneous lymphocytic leukemia in a cat. Cornell Vet. 1967 Apr;57(2):302–307. [PubMed] [Google Scholar]
  20. Rickard C. G., Post J. E., Noronha F., Barr L. M. A transmissible virus-induced lymphocytic leukemia of the cat. J Natl Cancer Inst. 1969 Jun;42(6):987–1014. [PubMed] [Google Scholar]
  21. Robinson H. L. Isolation of noninfectious particles containing Rous sarcoma virus RNA from the medium of Rous sarcoma virus-transformed nonproducer cells. Proc Natl Acad Sci U S A. 1967 Jun;57(6):1655–1662. doi: 10.1073/pnas.57.6.1655. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. SARMA P. S., TURNER H. C., HUEBNER R. J. AN AVIAN LEUCOSIS GROUP-SPECIFIC COMPLEMENT FIXATION REACTION. APPLICATION FOR THE DETECTION AND ASSAY OF NON-CYTOPATHOGENIC LEUCOSIS VIRUSES. Virology. 1964 Jul;23:313–321. doi: 10.1016/0042-6822(64)90253-3. [DOI] [PubMed] [Google Scholar]
  23. Sarma P. S., Cheong M. P., Hartley J. W., Huebner R. J. A viral interference test for mouse leukemia viruses. Virology. 1967 Sep;33(1):180–184. doi: 10.1016/0042-6822(67)90111-0. [DOI] [PubMed] [Google Scholar]
  24. Sarma P. S., Log T., Gilden R. V. Studies of hamster-specific oncogenic virus derived from hamster tumors induced by Kirsten murine sarcoma virus. Proc Soc Exp Biol Med. 1970 Feb;133(2):718–722. doi: 10.3181/00379727-133-34551. [DOI] [PubMed] [Google Scholar]
  25. Sarma P. S., Vass W., Huebner R. J. Evidence for the in vitro transfer of defective Rous sarcoma virus genome from hamster tumor cells to chick cells. Proc Natl Acad Sci U S A. 1966 Jun;55(6):1435–1442. doi: 10.1073/pnas.55.6.1435. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Ting R. C. Biological and serological properties of viral particles from a nonproducer rat neoplasm induced by murine sarcoma virus (Moloney). J Virol. 1968 Sep;2(9):865–868. doi: 10.1128/jvi.2.9.865-868.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Vogt P. K. A virus released by "nonproducing" Rous sarcoma cells. Proc Natl Acad Sci U S A. 1967 Sep;58(3):801–808. doi: 10.1073/pnas.58.3.801. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Vogt P. K., Sarma P. S., Huebner R. J. Presence of avian tumor virus group-specific antigen in nonproducing Rous sarcoma cells of the chicken. Virology. 1965 Oct;27(2):233–236. doi: 10.1016/0042-6822(65)90168-6. [DOI] [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES