Abstract
The addition of vinblastine to high-speed supernatants derived from homogenates of cultured mouse neuroblastoma cells results in the formation of a precipitate which has been characterized as microtubule protein by the following criteria: colchicine-binding activity, molecular weight, amino acid composition, and electrophoretic mobility. The method therefore permits the rapid isolation of microtubule protein from crude supernatants of neuroblastoma cells.
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- Bensch K. G., Malawista S. E. Microtubular crystals in mammalian cells. J Cell Biol. 1969 Jan;40(1):95–107. doi: 10.1083/jcb.40.1.95. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Bensch K. G., Marantz R., Wisniewski H., Shelanski M. Induction in vitro of microtubular crystals by vinca alkaloids. Science. 1969 Aug 1;165(3892):495–496. doi: 10.1126/science.165.3892.495. [DOI] [PubMed] [Google Scholar]
- Borisy G. G., Taylor E. W. The mechanism of action of colchicine. Binding of colchincine-3H to cellular protein. J Cell Biol. 1967 Aug;34(2):525–533. doi: 10.1083/jcb.34.2.525. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Brinkley B. R., Murphy P., Richardson L. C. Procedure for embedding in situ selected cells cultured in vitro. J Cell Biol. 1967 Oct;35(1):279–283. doi: 10.1083/jcb.35.1.279. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Creasey W. A., Chou T. C. The binding of colchicine by sarcoma 180 cells. Biochem Pharmacol. 1968 Mar;17(3):477–480. doi: 10.1016/0006-2952(68)90259-1. [DOI] [PubMed] [Google Scholar]
- Gray R. H., Steffensen D. M. A high-resolution method for slicing and counting radioactivity in polyacrylamide gels. Anal Biochem. 1968 Jul;24(1):44–53. doi: 10.1016/0003-2697(68)90058-4. [DOI] [PubMed] [Google Scholar]
- LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
- Malawista S. E., Sato H. Vinblastine produces uniaxial, birefringent crystals in starfish oocytes. J Cell Biol. 1969 Aug;42(2):596–599. doi: 10.1083/jcb.42.2.596. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Marantz R., Ventilla M., Shelanski M. Vinblastine-induced precipitation of microtubule protein. Science. 1969 Aug 1;165(3892):498–499. doi: 10.1126/science.165.3892.498. [DOI] [PubMed] [Google Scholar]
- Schochet S. S., Jr, Lampert P. W., Earle K. M. Neuronal changes induced by intrathecal vincristine sulfate. J Neuropathol Exp Neurol. 1968 Oct;27(4):645–658. [PubMed] [Google Scholar]
- Shapiro A. L., Viñuela E., Maizel J. V., Jr Molecular weight estimation of polypeptide chains by electrophoresis in SDS-polyacrylamide gels. Biochem Biophys Res Commun. 1967 Sep 7;28(5):815–820. doi: 10.1016/0006-291x(67)90391-9. [DOI] [PubMed] [Google Scholar]
- Shelanski M. L., Taylor E. W. Properties of the protein subunit of central-pair and outer-doublet microtubules of sea urchin flagella. J Cell Biol. 1968 Aug;38(2):304–315. doi: 10.1083/jcb.38.2.304. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Stephens R. E. On the structural protein of flagellar outer fibers. J Mol Biol. 1968 Mar 14;32(2):277–283. doi: 10.1016/0022-2836(68)90009-0. [DOI] [PubMed] [Google Scholar]
- Weisenberg R. C., Borisy G. G., Taylor E. W. The colchicine-binding protein of mammalian brain and its relation to microtubules. Biochemistry. 1968 Dec;7(12):4466–4479. doi: 10.1021/bi00852a043. [DOI] [PubMed] [Google Scholar]
- Wisniewski H., Shelanski M. L., Terry R. D. Effects of mitotic spindle inhibitors on neurotubules and neurofilaments in anterior horn cells. J Cell Biol. 1968 Jul;38(1):224–229. doi: 10.1083/jcb.38.1.224. [DOI] [PMC free article] [PubMed] [Google Scholar]