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. 1970 Jan;65(1):145–151. doi: 10.1073/pnas.65.1.145

Electron Spin Resonance Studies of Lipid-Protein Interaction in Human Serum Lipoproteins

A M Gotto 1,2, H Kon 1,2, M E Birnbaumer 1,2
PMCID: PMC286203  PMID: 4313193

Abstract

Spin-labeled derivatives of high density (HDL) and low density (LDL) lipoproteins from human serum were prepared with N-(1-oxyl-2,2,6,6-tetramethyl-4-piperidinyl)-maleimide. At the low ratios of spin label to protein employed, no changes in circular dichroism or immunochemical reactions were detected. The lipoprotein derivatives, labeled in their protein moieties, exhibited electron spin resonance spectra containing signals of strongly immobilized and weakly immobilized components. The strongly immobilized component was relatively more prominent in high density than in low density lipoproteins. Delipidation or addition of detergent greatly reduced the relative magnitude of the highly-immobilized signal. Reaction with specific antibodies did not alter the signals of spin-labeled lipoproteins or apoproteins. The signals of the reconstituted complex of spin-labeled apoHDL-phospholipid were indistinguishable from those of a spin-labeled apoHDL in the absence of lipid.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Barratt M. D., Green D. K., Chapman D. Spin-labelled lipid-protein complexes. Biochim Biophys Acta. 1968 Jan 10;152(1):20–27. doi: 10.1016/0005-2760(68)90004-0. [DOI] [PubMed] [Google Scholar]
  2. Chapman D., Leslie R. B., Hirz R., Scanu A. M. 220 MHz nuclear magnetic resonance spectra of high density serum lipoproteins. Nature. 1969 Jan 18;221(5177):260–261. doi: 10.1038/221260a0. [DOI] [PubMed] [Google Scholar]
  3. Chapman D., Leslie R. B., Hirz R., Scanu A. M. High-resolution NMR spectra of high-density serum lipoproteins. Biochim Biophys Acta. 1969 Apr 29;176(3):524–536. doi: 10.1016/0005-2760(69)90219-7. [DOI] [PubMed] [Google Scholar]
  4. Gotto A. M., Levy R. I., Fredrickson D. S. Human serum beta-lipoprotein: preparation and properties of a delipidated, soluble derivative. Biochem Biophys Res Commun. 1968 Apr 19;31(2):151–157. doi: 10.1016/0006-291x(68)90722-5. [DOI] [PubMed] [Google Scholar]
  5. Gotto A. M., Levy R. I., Fredrickson D. S. Observations on the conformation of human beta lipoprotein: evidence for the occurrence of beta structure. Proc Natl Acad Sci U S A. 1968 Aug;60(4):1436–1441. doi: 10.1073/pnas.60.4.1436. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Gotto A. M., Levy R. I., Lindgren F. T., Fredrickson D. S. Partial delipidation of human serum beta-lipoprotein by sodium decyl sulfate. Biochim Biophys Acta. 1969 Apr 29;176(3):667–669. doi: 10.1016/0005-2760(69)90241-0. [DOI] [PubMed] [Google Scholar]
  7. Gotto A. M., Levy R. I., Rosenthal A. S., Birnbaumer M. E., Fredrickson D. S. The structure and properties of human beta-lipoprotein and beta-apoprotein. Biochem Biophys Res Commun. 1968 Jun 10;31(5):699–705. doi: 10.1016/0006-291x(68)90618-9. [DOI] [PubMed] [Google Scholar]
  8. Gotto A. M., Levy R. I., Rosenthal A. S., Fredrickson D. S. Human serum beta-lipoprotein. Nature. 1968 Sep 14;219(5159):1157–1159. doi: 10.1038/2191157a0. [DOI] [PubMed] [Google Scholar]
  9. Griffith O. H., McConnell H. M. A nitroxide-maleimide spin label. Proc Natl Acad Sci U S A. 1966 Jan;55(1):8–11. doi: 10.1073/pnas.55.1.8. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Griffith O. H., McConnell H. M. A nitroxide-maleimide spin label. Proc Natl Acad Sci U S A. 1966 Jan;55(1):8–11. doi: 10.1073/pnas.55.1.8. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. HAVEL R. J., EDER H. A., BRAGDON J. H. The distribution and chemical composition of ultracentrifugally separated lipoproteins in human serum. J Clin Invest. 1955 Sep;34(9):1345–1353. doi: 10.1172/JCI103182. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. LEVY R. I., FREDRICKSON D. S. HETEROGENEITY OF PLASMA HIGH DENSITY LIPOPROTEINS. J Clin Invest. 1965 Mar;44:426–441. doi: 10.1172/JCI105156. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  14. Levy R. I., Lees R. S., Fredrickson D. S. The nature of pre beta (very low density) lipoproteins. J Clin Invest. 1966 Jan;45(1):63–77. doi: 10.1172/JCI105324. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Margolis S., Langdon R. G. Studies on human serum beta-1-lipoprotein. II. Chemical modifications. J Biol Chem. 1966 Jan 25;241(2):477–484. [PubMed] [Google Scholar]
  16. Sandberg H. E., Piette L. H. EPR studies of psychotropic drug interactions at cell membranes. Agressologie. 1968 Jan-Feb;9(1):59–67. [PubMed] [Google Scholar]
  17. Scanu A. M. On the temperature dependence of the conformation of human serum high density lipoprotein. Biochim Biophys Acta. 1969 May;181(1):268–274. doi: 10.1016/0005-2795(69)90249-9. [DOI] [PubMed] [Google Scholar]
  18. Scanu A. Binding of human serum high density lipoprotein apoprotein with aqueous dispersions of phospholipids. J Biol Chem. 1967 Feb 25;242(4):711–719. [PubMed] [Google Scholar]
  19. Scanu A., Hirz R. On the structure of human serum high-density lipoprotein: studies by the technique of circular dichroism. Proc Natl Acad Sci U S A. 1968 Mar;59(3):890–894. doi: 10.1073/pnas.59.3.890. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Scanu A., Pollard H., Hirz R., Kothary K. On the conformational instability of human serum low-density lipoprotein: effect of temperature. Proc Natl Acad Sci U S A. 1969 Jan;62(1):171–178. doi: 10.1073/pnas.62.1.171. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Sharpless N. E., Flavin M. The reactions of amines and amino acids with maleimides. Structure of the reaction products deduced from infrared and nuclear magnetic resonance spectroscopy. Biochemistry. 1966 Sep;5(9):2963–2971. doi: 10.1021/bi00873a028. [DOI] [PubMed] [Google Scholar]
  22. Steim J. M., Edner O. J., Bargoot F. G. Structure of human serum lipoproteins: nuclear magnetic resonance supports a micellar model. Science. 1968 Nov 22;162(3856):909–911. doi: 10.1126/science.162.3856.909. [DOI] [PubMed] [Google Scholar]
  23. VAERMAN J. P., HEREMANS J. F., VAERMAN C. STUDIES OF THE IMMUNE GLOBULINS OF HUMAN SERUM. I. A METHOD FOR THE SIMULTANEOUS ISOLATION OF THE TREE IMMUNE GLOBULINS (GAMMA-SS, GAMMA-IM AND GAMMA-1A) FROM INDIVIDUAL SMALL SERUM SAMPLES. J Immunol. 1963 Jul;91:7–10. [PubMed] [Google Scholar]
  24. Waggoner A. S., Griffith O. H., Christensen C. R. Magnetic resonance of nitroxide probes in micelle-containing solutions. Proc Natl Acad Sci U S A. 1967 May;57(5):1198–1205. doi: 10.1073/pnas.57.5.1198. [DOI] [PMC free article] [PubMed] [Google Scholar]

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