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Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1970 Jan;65(1):152–159. doi: 10.1073/pnas.65.1.152

Changes in the Physical State of DNA during Replication in Regenerating Liver of the Rat*

Henry Berger Jr 1,†, J Logan Irvin 1
PMCID: PMC286204  PMID: 5263745

Abstract

Evidence is presented which demonstrates that DNA synthesis in regenerating liver is discontinuous. In pulse-labeling experiments from 30 seconds to 10 minutes, the earliest detectable intermediate in DNA replication appears to be double-stranded DNA which has a significant degree of single-stranded character probably due to the existence of gaps in the newly synthesized strand. The label begins to move into completely double-stranded DNA 10-30 minutes after the commencement of labeling. The average molecular weight of the single-stranded „unit” of DNA replication is determined to be approximately 7.5 million. Smaller units may be produced but not observed as a result of imperfect synchronization of DNA synthesis.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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