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. 1970 Jan;65(1):226–233. doi: 10.1073/pnas.65.1.226

A Study on the Transcription of the Polyoma Viral Genome*

J Hudson 1,†, D Goldstein 1,‡, R Weil 1
PMCID: PMC286214  PMID: 4313194

Abstract

The transcription of polyoma (Py) viral RNA was studied in primary mouse kidney cell cultures (a) during normal, asynchronous infection, (b) under conditions where Py-induced DNA synthesis was inhibited with 5-fluorodeoxyuridine, and (c) after the synchronized onset of Py-induced DNA synthesis. The results obtained suggest that the bulk of Py RNA transcribed after the (asynchronous or synchronized) onset of Py-induced DNA synthesis, designated as „late” Py RNA, corresponds to the polycistronic transcript of most or all of the genetic information contained in a strand of circular Py DNA. The rate at which late Py RNA is transcribed is related to the amount of free Py DNA present in the cultures. Py-infected cultures contain a second, minor component of Py RNA (of one or possibly more species), designated as „early” Py RNA. At least some of this RNA continues to be transcribed if Py-induced DNA synthesis is inhibited with 5-fluorodeoxyuridine and, possibly, also late during normal infection.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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