Figure 3. Cell-based reconstitution of the AIM2 inflammasome.

a, Percentages of ASC pyroptosomes in untransfected (none) or poly (dA:dT)-transfected THP-1 cells. Values represent mean and s.d. (n = 3). b, Fluorescence confocal images showing formation of the speck-like ASC pyroptosomes only in 293-AIM2-ASC-EGFP-N1 cells (right panels), but not in 293-ASC-EGFP-N1 cells (left panels), 2 h after transfection with vehicle (control, upper panels) or poly (dA:dT) (lower panels). DIC, Differential Interference Contrast. c, Percentages of ASC pyroptosomes in the indicated cell lines following transfection with poly (dA:dT) for 2h. Values represent mean ± S.D. (n = 7); *, P<0.005; **, P<0.001. d, Immunoblot for caspase-1 in lysates from 293-caspase-1-ASC (1^st to 5^th lanes) or 293-caspase-1-ASC-AIM2 (6^th to 10^th lanes) cells following transfection with the indicated types of DNA (0.5 µg/1 × 10⁶ cells) for 24 h. e, Immunoblot for AIM2 (upper panel) or Omi (lower panel) in lysates from uninduced (1^st lane) or interferon γ-induced (2^nd lane) THP-1 cells, or 293-caspase-1-ASC-AIM2 cells (3^rd lane). f, Immunoblot for caspase-1 in lysates from 293-caspase-1-ASC (1^st to 4^th lanes) or 293-caspase-1-ASC-NALP3 (5^th to 8^th lanes) cells following transfection with the indicated plasmid DNA (0.5 µg /1 × 10⁶ cells) for 24 h.