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. 1989 Jan;86(1):65–69. doi: 10.1073/pnas.86.1.65

Copper-induced binding of cellular factors to yeast metallothionein upstream activation sequences.

J M Huibregtse 1, D R Engelke 1, D J Thiele 1
PMCID: PMC286404  PMID: 2643107

Abstract

Copper-inducible transcription of the yeast metallothionein gene (CUP1) occurs by means of cis-acting upstream activation sequences (UAS) and trans-acting cellular factors. We have used a high-resolution chromosomal footprinting technique to detect the interaction of cellular factors with UASCUP1. Our results demonstrate that one or more cellular factors bind to UASCUP1 in a copper-inducible fashion. This copper-inducible binding is enhanced in a yeast strain that harbors several copies of the positive regulatory gene ACE1 and is not detectable in yeast cells that contain a nonfunctional (ace1-delta 1) locus. The correlation between yeast metallothionein gene activation and copper-inducible occupation of UASCUP1 sequences suggests that the binding of metallothionein transcriptional regulatory factors to cis-acting control sequences is copper-inducible.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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