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Sedimentation velocity analyses of wild-type RePC and the mutants R548K, R548Q, R548A, Q552N and Q552A. Experiments were performed as described in Materials and Methods. Plots of derived sedimentation coefficient distribution (c(s)) vs. sedimentation coefficient obtained from the application of SEDFIT and maximum entropy methods of analysis to the original apparent sedimentation coefficient distribution vs. apparent sedimentation coefficient data (g(s*) vs. s* plots).
