Figure 6.

HIF-induced CXCL12 stimulates in vivo angiogenesis. (A) CXCL12-, HIF-1α- or HIF-2α-over-expressing LP-1 cells were injected subcutaneously in a Matrigel plug into mice (n=12/group), and half of the mice were administered the CXCR4 antagonist, T140. After 2 weeks, mice were euthanized and macroscopic photographs were taken of the implants. (B) The hemoglobin content of implants from untreated (white bars) and T140-treated (black bars) mice were measured and normalized to cell burden as described in the Design and Methods sections. Columns, mean (n=6/group); bars, SEM. *P<0.05, compared to vector control.