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. Author manuscript; available in PMC: 2010 May 6.
Published in final edited form as: Oncogene. 2009 Oct 5;29(1):117–127. doi: 10.1038/onc.2009.311

Figure 4. Effects of serum deprivation, cell density and weak base treatments on Hsc70 secretion.

Figure 4

Control cells (open circle) and Cath D expressing clones (filled circle) were cultured for 2 days in the presence of the indicated percentage of foetal calf serum. Hsc70 concentrations within the cells (A) and in the corresponding culture media (B) were evaluated by Western blot using Hsc70 (100 ng/track) as protein loading control. Hsc70 secretion was significantly different (p = 0.0014) between control and over-expressing cath D cells. (C) Effects of cell density on Hsc70 secretion was analyzed in the range from 0.5 to 2 106 cells/cm2. (p=0.0056) (D) Regulation of Hsc70 secretion by cath D and lysosomotropic bases. Cath D cells were cultured for 5 days in serum-free medium, either untreated (control) or treated with 50 μM chloroquine (CQ) or 0.2 mM ammonium chloride (NH4Cl). Hsc70 was assayed in the culture medium and expressed as ng/106 cells/24 h, and Western blot of the corresponding conditioned media was shown.