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. Author manuscript; available in PMC: 2010 Nov 6.
Published in final edited form as: Nature. 2010 Apr 4;465(7294):91–95. doi: 10.1038/nature09011

Figure 3.

Figure 3

Ectopic expression of ppk28 confers water sensitivity. a. Extracellular bristle recordings of i-type sensilla (non-water responsive) from Gr66a-Gal4 flies lacking (-) or containing (+) UAS-ppk28 upon water, 0.5M NMDG, 1M NMDG or 0.01M caffeine stimulation (at recording). b. Scatter plot of water responses (mean ± s.e.m in bars; data points are dots) and summary of all responses (mean ± s.e.m.). Responses are different to water (***P=2.52E-16) and 0.5M NMDG (*P=0.016) (t-test; n=7-27). c. G-CaMP fluorescence increase in Gr66a bitter-sensing projections (left) and Gr66a projections expressing ppk28 (right), after water stimulation (%maxΔF/F) (SOG, scale 50μm). d. Responses in Gr66a cells (left) and Gr66a cells expressing ppk28 (right) to water (at arrow). e. Summary of fluorescence changes in Gr66a cells without (grey) or with ppk28 (green) tested with water, 0.5 and 1M NMDG and 0.5, 1 and 2M sucrose. (n=4-5 trials/concentration ± s.e.m; t-test, versus Gr66a control, water: **P=0.013, 0.5M NMDG: *P=0.03; water: **P=0.002, 0.5M sucrose: ***P=0.0002).