Figure 1.

Significant inhibition of ESC-like gene sets with Meis1 knockdown in murine MLL-AF9 leukemia. Expression of gene sets composed of core-ESC module, core-ESC module without proliferation-associated genes,^4,6 and another ESC-like gene set⁷ were compared across the dataset of control and Meis1-shRNA transduced murine MLL-AF9 leukemia cells using GSEA. Shown are the enrichment plots of GSEA analysis illustrating that the gene sets composed of core ESC module (A), core ESC module without the genes associated with proliferation (B), and the ECS-like signature (C) were all significantly inhibited with Meis1 knockdown (preranked analysis using Log₂ fold change statistic, 1000 permutations, P < .001 for all analyses; results were similar when the paired t test statistic was used).