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. 1989 Mar;86(5):1652–1656. doi: 10.1073/pnas.86.5.1652

Nonterminal differentiation represses the neoplastic phenotype in spontaneously and simian virus 40-transformed cells.

R E Scott 1, D N Estervig 1, C Y Tzen 1, P Minoo 1, P B Maercklein 1, B J Hoerl 1
PMCID: PMC286757  PMID: 2537979

Abstract

The potential of nonterminal cellular differentiation to stably repress the expression of the neoplastic phenotype of transformed cells is established. Nonterminal differentiation induces spontaneously transformed 3T3 T cells to revert to a nontransformed state and induces the revertant cell clones to become resistant to retransformation by UV irradiation or 4-nitroquinoline oxide treatment. Nonterminal differentiation also induces simian virus 40-transformed 3T3 T cells to repress expression of the large tumor antigen and to revert to a nontransformed state. Although the molecular mechanisms that mediate these and other forms of anticancer activity have not been definitively established, data are presented which suggest that differentiation-induced repression of large tumor antigen expression can be regulated at the level of transcription and/or RNA processing.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Anders A., Anders F. Etiology of cancer as studied in the platyfish-swordtail system. Biochim Biophys Acta. 1978 Sep 18;516(1):61–95. doi: 10.1016/0304-419x(78)90004-5. [DOI] [PubMed] [Google Scholar]
  2. DiPaolo J. A. Relative difficulties in transforming human and animal cells in vitro. J Natl Cancer Inst. 1983 Jan;70(1):3–8. [PubMed] [Google Scholar]
  3. Friend S. H., Bernards R., Rogelj S., Weinberg R. A., Rapaport J. M., Albert D. M., Dryja T. P. A human DNA segment with properties of the gene that predisposes to retinoblastoma and osteosarcoma. Nature. 1986 Oct 16;323(6089):643–646. doi: 10.1038/323643a0. [DOI] [PubMed] [Google Scholar]
  4. Gootwine E., Webb C. G., Sachs L. Participation of myeloid leukaemic cells injected into embryos in haematopoietic differentiation in adult mice. Nature. 1982 Sep 2;299(5878):63–65. doi: 10.1038/299063a0. [DOI] [PubMed] [Google Scholar]
  5. Gorman C. M., Moffat L. F., Howard B. H. Recombinant genomes which express chloramphenicol acetyltransferase in mammalian cells. Mol Cell Biol. 1982 Sep;2(9):1044–1051. doi: 10.1128/mcb.2.9.1044. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Graham F. L., van der Eb A. J. A new technique for the assay of infectivity of human adenovirus 5 DNA. Virology. 1973 Apr;52(2):456–467. doi: 10.1016/0042-6822(73)90341-3. [DOI] [PubMed] [Google Scholar]
  7. Krawisz B. R., Scott R. E. Coupling of proadipocyte growth arrest and differentiation. I. Induction by heparinized medium containing human plasma. J Cell Biol. 1982 Aug;94(2):394–399. doi: 10.1083/jcb.94.2.394. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Pierce G. B. The cancer cell and its control by the embryo. Rous-Whipple Award lecture. Am J Pathol. 1983 Oct;113(1):117–124. [PMC free article] [PubMed] [Google Scholar]
  9. Podesta A. H., Mullins J., Pierce G. B., Wells R. S. The neurula stage mouse embryo in control of neuroblastoma. Proc Natl Acad Sci U S A. 1984 Dec;81(23):7608–7611. doi: 10.1073/pnas.81.23.7608. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Samid D., Flessate D. M., Friedman R. M. Interferon-induced revertants of ras-transformed cells: resistance to transformation by specific oncogenes and retransformation by 5-azacytidine. Mol Cell Biol. 1987 Jun;7(6):2196–2200. doi: 10.1128/mcb.7.6.2196. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Scott R. E., Boone C. W. PRomotion of smooth surface tumorigenicity by phorbol myristate acetate in Balb/3T3 cells and Balb/3T3 T proadipocytes. J Natl Cancer Inst. 1981 Apr;66(4):733–736. [PubMed] [Google Scholar]
  12. Scott R. E., Florine D. L., Wille J. J., Jr, Yun K. Coupling of growth arrest and differentiation at a distinct state in the G1 phase of the cell cycle: GD. Proc Natl Acad Sci U S A. 1982 Feb;79(3):845–849. doi: 10.1073/pnas.79.3.845. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Scott R. E., Hoerl B. J., Wille J. J., Jr, Florine D. L., Krawisz B. R., Yun K. Coupling of proadipocyte growth arrest and differentiation. II. A cell cycle model for the physiological control of cell proliferation. J Cell Biol. 1982 Aug;94(2):400–405. doi: 10.1083/jcb.94.2.400. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Southern P. J., Berg P. Transformation of mammalian cells to antibiotic resistance with a bacterial gene under control of the SV40 early region promoter. J Mol Appl Genet. 1982;1(4):327–341. [PubMed] [Google Scholar]
  15. Sparks R. L., Seibel-Ross E. I., Wier M. L., Scott R. E. Differentiation, dedifferentiation, and transdifferentiation of BALB/c 3T3 T mesenchymal stem cells: potential significance in metaplasia and neoplasia. Cancer Res. 1986 Oct;46(10):5312–5319. [PubMed] [Google Scholar]
  16. Stanbridge E. J., Der C. J., Doersen C. J., Nishimi R. Y., Peehl D. M., Weissman B. E., Wilkinson J. E. Human cell hybrids: analysis of transformation and tumorigenicity. Science. 1982 Jan 15;215(4530):252–259. doi: 10.1126/science.7053574. [DOI] [PubMed] [Google Scholar]
  17. Terzaghi M., Little J. B. X-radiation-induced transformation in a C3H mouse embryo-derived cell line. Cancer Res. 1976 Apr;36(4):1367–1374. [PubMed] [Google Scholar]
  18. Wille J. J., Jr, Scott R. E. Suppression of tumorigenicity by the cell-cycle-dependent control of cellular differentiation and proliferation. Int J Cancer. 1986 Jun 15;37(6):875–881. doi: 10.1002/ijc.2910370613. [DOI] [PubMed] [Google Scholar]

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